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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   7

Issue No. :  8

Year :  2014

Pages :   897-901

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Rapid RP-HPLC Method for Quantitative Determination of Emtricitabine in Bulk and Pharmaceutical Dosage Form



Address:   K. Vijaya Sri, M. Madhuri, G. Vinay Jain and K. Suresh
Department of Pharmaceutical Analysis, Malla Reddy College of Pharmacy, Maisammaguda, Secunderabad-500 014 Andhra Pradesh, India
*Corresponding Author
DOI No: Not Available

ABSTRACT:
A simple, rapid, sensitive, linear, precise, accurate, rugged and robust RP-HPLC-PDA method was developed and validated for the determination of emtricitabine in bulk and capsule dosage form. Reversed-phase chromatography was performed on BDS hypersil C-18 (250 mm × 4.6 mm, 5 micrometer) column of Waters HPLC with Empower software. Methanol: water: acetonitrile 80:13.5:6.5 (v/v/v) was used as mobile phase at a flow rate of 1 mL min-1 with PDA detection at 239 nm. The retention time of emtricitabine was 2.78 min. Linearity was observed in the concentration range of 2-200 microgram mL-1 with regression equation y = 53699x + 122068 (R2 = 0.9998). The method was validated as per ICH guidelines. The %RSD for intra-day and inter-day precision were found to be less than 1 %. The percentage assay of emtricitabine was 100.44% and the percentage recovery was in good agreement with the labelled amount in the pharmaceutical formulation. The proposed method is simple, rapid, sensitive, linear, precise, accurate, robust and rugged and hence applied for the determination of emtricitabine in bulk and pharmaceutical dosage form.
KEYWORDS:
Emtricitabine; RP-HPLC-PDA; Method validation; Capsules, ICH guidelines.
Cite:
K. Vijaya Sri, M. Madhuri, G. Vinay Jain and K. Suresh. Rapid RP-HPLC Method for Quantitative Determination of Emtricitabine in Bulk and Pharmaceutical Dosage Form. Research J. Pharm. and Tech. 7(8): August 2014 Page 897-901.
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