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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   7

Issue No. :  6

Year :  2014

Pages :   650-654

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Simultaneous RP-HPLC estimation of Cinnarizine and Domperidone in Tablet



Address:   T. M. Kalyankar1*, P D Kulkarni1, P. P. Panchakshari1, A. S. Narute2
1School of Pharmacy, Swami Ramanand Teerth Marathwada University, Vishnupuri, Nanded-431606. (MS), India.
2Yash Institute of Pharmacy, Aurangabad (MS), India.
*Corresponding Author
DOI No: Not Available

ABSTRACT:
A simple reversed-phase high-performance liquid chromatographic method has been developed and validated for simultaneous estimation of Cinnarizine and Domperidone in tablets. The compounds were separated on a ‘Hypersil C18’ analytical column. The mobile phase used for separation was methanol: 0.05M phosphate buffer in the ratio (80:20 v/v). The pH was adjusted to 3.8 with ortho phosphoric acid. The flow rate was maintained to 1 ml/min. UV detection was performed at 275 nm. The method was validated for accuracy, precision, specificity, linearity, and sensitivity. Total chromatographic analysis time per sample was 10 min. The retention time for Cinnarizine and Domperidone was found to be 7.33 and 3.14 min respectively. Validation studies revealed that the method is specific, rapid, reliable, and reproducible. Calibration plots were linear over the concentration ranges of 10-35 microgram/mLand 5-30 microgram/mLfor Cinnarizine and Domperidone respectively. The limit of detection and quantitation was 0.067 microgram/ml and 0.221 microgram/ml for Cinnarizine and 0.051 microgram/ml and 0.169 microgram/ml for Domperidone respectively. The high recovery and low relative standard deviation confirm the suitability of the method for determination of Cinnarizine and Domperidone in tablets.
KEYWORDS:
Cinnarizine, Domperidone, RP-HPLC, Validation, Tablet dosage form.
Cite:
T. M. Kalyankar, P D Kulkarni, P. P. Panchakshari, A. S. Narute. Simultaneous RP-HPLC estimation of Cinnarizine and Domperidone in Tablet. Research J. Pharm. and Tech. 7(6): June, 2014; Page 650-654.
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