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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   7

Issue No. :  5

Year :  2014

Pages :   537-543

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Antimicrobial evaluation and quantification of apigenin content by HPTLC in methanol stem extract of Cardiospermum halicacabum L



Address:   A. Rajasekaran*, R. Arivukkarasu, Joyce Mathew P, Raja Bonagiri, Ramadasu Partha Saradhi
KMCH College of Pharmacy, Coimbatore, Tamil Nadu
*Corresponding Author
DOI No: Not Available

ABSTRACT:
The present study was aimed to evaluate the anti-microbial activity of methanolic stem extract of Cardiospermum halicacabum (MSECH) and to develop and validate a HPTLC method for quantification of apigenin in MSECH. The in vitro anti-microbial efficacy of MESCH was evaluated by disc diffusion method and minimum inhibitory concentration against six bacterial strains Staphylococcus aureus, Bacillus lentius, Bacillus subtilis, Klebsiella pneumonia, Pseudomonas aeruginosa, Escherichia coli and three fungal strains Aspergillus niger, Aspergillus fumigatus and Candida albicans. Gram positive organism Bacillus lentius and Gram negative organism Pseudomonas aeruginosa was found to be most susceptible bacterial strains. Significant antifungal activity was observed for MSECH against Candida albicans and Aspergillus fumigatus. The results of the present investigation indicated that MSECH possess anti-microbial activity which may be due to the presence of flavonoid apigenin. The linearity and correlation coefficient was found to be 100-1000 ng/spot and 0.9962 respectively for the quantification of apigenin in HPTLC method. Percentage recovery, LOD and LOQ found to be 98.64%, 140.46 ng/spot and 426.34 ng/spot respectively.
KEYWORDS:
Cardiospermum halicacabum, anti-microbial activity, stem extract, apigenin.
Cite:
A. Rajasekaran, R. Arivukkarasu, Joyce Mathew P, Raja Bonagiri, Ramadasu Partha Saradhi. Antimicrobial evaluation and quantification of apigenin content by HPTLC in methanol stem extract of Cardiospermum halicacabum L. Research J. Pharm. and Tech. 7(5): May, 2014; Page 537-543.
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