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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   7

Issue No. :  5

Year :  2014

Pages :   526-530

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Production of L-Asparaginase by Marine Actinobacteria Isolated from the Rhizosphere of Mangrove Plants



Address:   Pitamber J, Hitender P, Pradeep S, S.R Sathish Kumar, Veena S, K.V. Bhaskara Rao*
Molecular and Microbiology Research Laboratory, Environmental Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore - 632 014, Tamil Nadu, India.
*Corresponding Author
DOI No: Not Available

ABSTRACT:
The aim of the present study was to isolate L-asparaginase producing actinobacteria from mangrove sediment. The samples were collected from Parangapattai coastal area of Tamilnadu, India. A total of 24 actinobacteria colonies were isolated, among them 14 strains were separated and selected based on colony morphology. The isolated actinobacterial strains were screened for L- Asparginase enzyme production on M9 medium. Out of 14 isolates, 4 isolates (VITPPH1, VITPPH6, VITPPH7 and VITPPH10) showed positive results in Rapid plate Assay method. All the four actinobacterial strains showed 25-60 µg ammonia/mL/h range of L-asparagines activity. VITPPH10 strain showed highest L-asparaginase activity (58 µg ammonia/mL/h). Among the 14 isolates, VITPPH10 isolate showed best antimicrobial activity against Salmonella typhi and Pseudomonas aeruginosa. Cytotoxic assay was determined by using brine shrimp assay. VITPPH10 showed 100% mortality within 24 hours. The potential actinobacterial strain VITPPH10 was identified as Streptomyces sp.
KEYWORDS:
L-asparaginase, Mangroves, Antimicrobial activity, Drug resistance bacteria, Cytotoxic assay.
Cite:
Pitamber J, Hitender P, Pradeep S, S.R Sathish Kumar, Veena S, K.V. Bhaskara Rao. Production of L-Asparaginase by Marine Actinobacteria Isolated from the Rhizosphere of Mangrove Plants. Research J. Pharm. and Tech. 7(5): May, 2014; Page 526-530.
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