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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   7

Issue No. :  5

Year :  2014

Pages :   521-525

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Cytotoxic Property of Cocos nucifera shell Extracts on HeLa Cells



Address:   NasimunIslam N1., Jemimah Naine S.2, Shaik Jameel2, S.S. Khora2, Venkat Kumar, Mohanasrinivasan V.2, C. Subathra Devi2, Panneerselvam A.1*
1Department of Zoology, Thiruvalluvar University, Vellore, Tamil Nadu, India
2School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, India.
*Corresponding Author
DOI No: Not Available

ABSTRACT:
The main objective was to investigate the cytotoxic properties of Cocos nucifera shell crude extract. The invitro antiproliferative activity against HeLa cells was measured by MTT assay which significantly decreased the number of HeLa cells by dose dependent manner with IC50 of 1.77mg/ml. However, the cytotoxic activity was found due to the apoptosis induced in HeLa cells, which was demonstrated by DNA fragmentation analysis. The expression of apoptosis related genes, p53 and Bcl2 were analyzed by RT PCR. The apoptosis triggered by Cocos nucifera shell extract in HeLa cells was associated with the downregulation of Bcl-2 gene expression. These findings demonstrate that the shell extracts of Cocos nucifera presents excellent cytotoxic properties with beneficial virtues.
KEYWORDS:
Bioassays, Apoptosis; Bcl-2, Cytotoxicity.
Cite:
NasimunIslam N1., Jemimah Naine S., Shaik Jameel, S.S. Khora, Venkat Kumar, Mohanasrinivasan V., C. Subathra Devi, Panneerselvam A. Cytotoxic Property of Cocos nucifera shell Extracts on HeLa Cells. Research J. Pharm. and Tech. 7(5): May, 2014; Page 521-525.
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