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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   7

Issue No. :  5

Year :  2014

Pages :   513-516

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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A novel method for determination of Tetracycline and its metabolite residues in cow milk



Address:   Tentu. Nageswara Rao1*, D. Sreenivasulu2, E.G. Sreenivasula Reddy3 and Kappari. Sharadha Devi4
1Department of Chemistry, Krishna University, Machilipatnam, Andhra Pradesh, India.
2Department of Chemistry, S.V. University, Tirupathi, Andhra Pradesh, India
3Department of Chemistry, S.K. University, Anantapur, Andhra Pradesh, India.
4Department of Pharmaceutics, GRY Institute of Pharmacy, Madhya Pradesh, India
*Corresponding Author
DOI No: Not Available

ABSTRACT:
A simple, sensitive and inexpensive method was developed using matrix solid-phase dispersion (MSPD), together with high performance liquid chromatographic method for determination of Tetracycline residues and its metabolite 4-Epitetracycline in cow milk. The evaluated parameters included the type and amount of sorbent (silica gel, C18 and alumina) and the nature of eluent (20% trichloro acetic acid , 0.01M citric acid, 0.01M disodium hydrogen phosphate, 0.01M EDTA and Methanol). The best results were obtained using 10 mL of milk sample, 1.0 g of C18 as sorbent and 20mL of 20% trichloroacetic acid , 0.01M citric acid, 0.01M disodium hydrogen phosphate, 0.01M EDTA and Methanol (1:2:2:2:3, (v/v)).The method was validated using in milk samples spiked with Tetracycline residues and its metabolite 4-Epitetracycline at different concentration levels (0.05 and 0.5 µg/mL). Average recoveries (using each concentration six replicates) ranged 87-96%, with relative standard deviations less than 2%, calibration solutions concentration in the range 0.05-5.0 µg/mL and limit of detection (LOD) and limit of quantification (LOQ) were 0.02 µg/mL and 0.05 µg/mL respectively.
KEYWORDS:
Matrix solid-phase dispersion, antibiotic residues, LOD and LOQ.
Cite:
Tentu. Nageswara Rao, D. Sreenivasulu, E.G. Sreenivasula Reddy, Kappari. Sharadha Devi. A novel method for determination of Tetracycline and its metabolite residues in cow milk. Research J. Pharm. and Tech. 7(5): May, 2014; Page 513-516.
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