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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   7

Issue No. :  4

Year :  2014

Pages :   433-438

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Talaromyces verruculosus, A Novel Marine Fungi as a Potent Polyhydroxybutyrate Degrader



Address:   S. Shalini Devi1, Y. Sreenivasulu2* and K.V. Bhaskar Rao1
1School of Biosciences and Technology, VIT University, Vellore 632 014,Tamilnadu, India.
2Watson Life Sciences, Tirupati-517 502, A.P, India.
*Corresponding Author
DOI No: Not Available

ABSTRACT:
Fungi were isolated from Marine water collected from three different regions of Andhra Pradesh, India. A total number of 45 fungi were isolated of which 9 showed varied sized clear zones on assay medium. Largest clear zone was detected below and around the fungal colony WFPHB2, T.verruculosus for which the PHB (Polyhydroxybutyrate) depolymerase productivity has not yet been published. This strain grew well on PDA (Potato Dextrose Agar) medium. In early stage of growth, enzyme production was highest at 30oC. Purification and characterization of PHB depolymerase secreted by WFPHB2 was carried which reported that single protein band of 45 kDa is responsible for depolymerase activity. 80┬Ál of the purified enzyme showed clear degradation zone during plate assay. 96 hrs incubation in liquid broth containing WFPHB2 resulted in 90%depolymerization of PHB films.
KEYWORDS:
Polyhydroxybutyrate depolymerase, T. verruculosus, marine fungi, novel fungi.
Cite:
S. Shalini Devi, Y. Sreenivasulu, K.V. Bhaskar Rao. Talaromyces verruculosus, A Novel Marine Fungi as a Potent Polyhydroxybutyrate Degrader. Research J. Pharm. and Tech. 7(4): April, 2014; Page 433-438.
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