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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   7

Issue No. :  11

Year :  2014

Pages :   1297-1299

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Optimization Studies on Nattokinase enzyme Production by a Mutant Strain Pseudomonas aeruginosa CMSS Isolated From Bovine Milk



Address:   C. Subathra Devi1*, Mohanasrinivasan. V2, Daria Das2, Pragya Sharma2, Vaishnavi. B2, Jemimah Naine. S2, Dhamodaran. D2
1Assistant Professor (Senior), Industrial Biotechnology Division, School of Biosciences and Technology, VIT University, Vellore- 632 014, Tamil Nadu, India
2School of Biosciences and Technology, Vellore Institute of Technology University, Vellore, Tamil Nadu, India.
*Corresponding Author
DOI No: Not Available

ABSTRACT:
Nattokinase is an enzyme with strong fibrinolytic activity to prevent thrombolytic diseases. Nattokinase is a serine protease; it founds much application in medical industry widely. Nattokinase was purified from the culture supernatant of mutant strain Pseudomonas aeuroginosa CMSS. The optimum pH and temperature for nattokinase production was found to be 6 and 25oC respectively. Production medium supplemented with 1 % sucrose and 1 % SSP showed maximum nattokinase yield and activity.
KEYWORDS:
Nattokinase, Pseudomonas aeruginosa CMSS, clot buster, optimization.
Cite:
C. Subathra Devi, Mohanasrinivasan. V, Daria Das, Pragya Sharma, Vaishnavi. B, Jemimah Naine. S, Dhamodaran. D. Optimization Studies on Nattokinase enzyme Production by a Mutant Strain Pseudomonas aeruginosa CMSS Isolated From Bovine Milk. Research J. Pharm. and Tech. 7(11): Nov. 2014 Page 1297-1299.
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