2nd International Conference on Fostering Interdisciplinary Research In Health Sciences (ICFIRHS) 2019 (01-May-2019)        |

Journal :   Research Journal of Pharmacy and Technology

Volume No. :   6

Issue No. :  9

Year :  2013

Pages :   985-989

ISSN Print :  0974-3618

ISSN Online :  0974-360X


Registration

Allready Registrered
Click to Login

Estimation of Gliclazide in Human Plasma by LCMS/MS



Address:   Srinivasa Reddy, Anandan Pattabi, Arvind Kumar, Sreedevi Rajpurohit, Arindam Mukhopadhyay* and Saral Thangam
Norwich Clinical Services Pvt. Ltd., 147/F, 8th Main, 3rd Block, Koramangala, Bangalore– 560034
*Corresponding Author
DOI No: Not Available

ABSTRACT:
Gliclazide and Glipizide (IS) were separated on a Reverse phase chromatography using the mobile phase mixture of ammonium acetate and methanol at a flow rate of 1.0 ml/min after extraction from plasma by liquid – liquid extraction method. Both the analyte and the IS were detected in API 4000 Mass spectrometer in the positive atmospheric pressure Ionization (API) mode with multiple reactions monitoring (MRM). The MRM transitions were monitored by following m/z for parent ion 324.1 and daughter ion 127.2 (Gliclazide), and m/z 446.1 and daughter 321.1 (Glipizide, IS). A linear calibration plot of Gliclazide was achieved in the concentration ranges of ranges of 7.776 ng/ml to 6000 ng/ml. Mean recovery was 75.67%.This method was fully validated for specificity, precision, accuracy, reproducibility and other criteria as per regulations.
KEYWORDS:
Gliclazide, Glipizide, Liquid-Liquid extraction, LCMS/MS, Human plasma, Validation.
Cite:
Srinivasa Reddy, Anandan Pattabi, Arvind Kumar, Sreedevi Rajpurohit, Arindam Mukhopadhyay, Saral Thangam. Estimation of Gliclazide in Human Plasma by LCMS/MS. Research J. Pharm. and Tech. 6(9): September 2013; Page 985-989.
[View HTML]      [View PDF]



Visitor's No. :   645737