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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   6

Issue No. :  9

Year :  2013

Pages :   1051-1057

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Amelioration and Affirmation for the Assessment of Curcumin in API and Ayurvedic Herbal Formulation Haridra Capsule by UFLC Discrete Method



Address:   Y. Thejeswari1*, S. Rubesh Kumar2
Department of Pharmaceutical Analysis, JNTUA - Oil Technological Research Institute, Anantapuram -515001, Andhra Pradesh, India.
*Corresponding Author
DOI No: Not Available

ABSTRACT:
Curcumin is an ancient medicine to present clinical trials. Curcuminoids natural yellow pigments, the principle constituents of Curcuma longa of Zingiberaceae family comprises of curcumin, demethoxycurcumin and bisdemethoxycurcumin. In the present study, a fast, simple and reliable method for the determination of curcumin present in the API and Ayurvedic formulation haridra capsule by using a single mobile phase for a cost effective method. The effluent was monitored at 419nm. The method was validated according to ICH guidelines various parameters like Specificity, Linearity, Precision, Accuracy, Limit of detection, Limit of quantification, Robustness and System suitability. The method was selected for the determination of curcumin. The system was linear with a correlation coefficient (r2) of 0.9998. RSDs% of intraday and interday precisions were determined to be 0.40 and 0.67 for curcumin. Limit of detection and limit of quantification was found to be as 2.97 and 9 for curcumin. The percentage recovery studies was found to be 99.47 to 102.2%w/v. Stress degradation studies proved that compound showed degradation in conditions like hydrolytic under acidic, alkaline, oxidative, photolytic and dry heat degradation studies for the API as per the ICH guidelines. The simplicity of the method makes it highly valuable for quantification of curcumin in API and Ayurvedic formulations.
KEYWORDS:
Curcumin, methanol, UFLC, isocratic elution, validation and stress degradation.
Cite:
Y. Thejeswari, S. Rubesh Kumar. Amelioration and Affirmation for the Assessment of Curcumin in API and Ayurvedic Herbal Formulation Haridra Capsule by UFLC Discrete Method. Amelioration and Affirmation for the Assessment of Curcumin in API and Ayurvedic Herbal Formulation Haridra Capsule by UFLC Discrete Method. Research J. Pharm. and Tech. 6(9): September 2013; Page 1051-1057.
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