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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   6

Issue No. :  8

Year :  2013

Pages :   889-893

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Exploration of alfa-amylase Inhibitor producing marine actinobacteria and evaluation of its antioxidant and antimicrobial activities –In vitro study



Address:   Ishita Chakraborty, Sathish Kumar S.R. and Bhaskara Rao K.V.*
Molecular and Microbiology Research Laboratory, Environmental Biotechnology Division, School of Bio-Sciences and Technology, VIT University, Vellore - 632 014, Tamil Nadu, India.
*Corresponding Author
DOI No: Not Available

ABSTRACT:
The purpose of this study is to explore the alfa-amylase enzyme inhibitor producing of actinobacteria from salt pan soil sample collected from coastal areas of Kothapattanam, Ongole, Andhra Pradesh, India. Among 28 colonies, only 3 acclimatized actinobacterial isolates (VITISB-3, VITISB-9 and VITISB-23) showed potency during primary screening technique. The isolates were inoculated in production media for secondary screening and bioactive compound extraction using different solvents of varying polarity. The ethyl acetate extracts of VITISB-23 and VITISB-3 isolates showed significant alfa-amylase inhibition activity while VITISB-9 showed less activity. These isolates were further screened for antimicrobial activity and free radical scavenging antioxidant potential. Among these isolates, VITISB-23 and VITISB-3 showed good antimicrobial activity while the other isolate, VITISB-9 showed moderate inhibition activity. VITISB-23 showed antimicrobial activity against all the pathogenic test organisms used in the study. In DPPH antioxidant assay, the isolate VITISB-23 showed a 96% free radical scavenging property, where VITISB-3 showed more than 80%. Based on these results we concluded that VITISB-23 possess potential biological activity and can be further studied for the isolation of new anti-diabetic compound and also for future commercial prospects in pharmaceutical industry for the formulation of medicinal drugs.
KEYWORDS:
Salt pan soil, Marine actinobacteria, Alfa-amylase inhibitor, antimicrobial activity, antioxidant activity.
Cite:
Ishita Chakraborty, Sathish Kumar S.R., Bhaskara Rao K.V. Exploration of alfa- amylase Inhibitor producing marine actinobacteria and evaluation of its antioxidant and antimicrobial activities –In vitro study. Research J. Pharm. and Tech. 6(8): August 2013; Page 889-893.
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