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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   6

Issue No. :  7

Year :  2013

Pages :   726-730

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Extractive Spectrophotometric Determination of Ofloxacin and Tinidazole with Bromophenol Blue.



Address:   Dr. Y. Haribabu, Mrs. Sheeja Velayudhan Kutty, Vidhya P.M.*,Greeshma S.
Grace College of Pharmacy, Palakkad, Kerala
*Corresponding Author
DOI No: Not Available

ABSTRACT:
The study aims to develop a simple, sensitive,rapid,accurate and precise extractive spectrophotometric method for the determination of ofloxacin and tinidazole in bulk drug and tablet dosage form. This method is based on the formation of yellow-ion pair complex between the drug and the anionic dye bromophenol blue in phthalate buffer at ph 3.6. The formed complexes were extracted with chloroform and measured at 426nm for ofloxacin – bromophenol blue and 312nm for tinidazole - bromophenol blue. Beer’s law was obeyed in the concentration range of 2-12µg/ml for ofloxacin and tinidazole with a linear regression value 0.994 and 0.996 respectively. Inter day and Intraday studies showed high degree of repeatability. Recoveries obtained do not differ significantly from 100% showed that there was no interference from the common excipients used in the tablet formulation indicating accuracy and reliability of the method.The proposed method was successfully applied for the determination of ofloxacin and tinidazole in commercial tablet containing 200 mg of ofloxacin and 300 mg of tinidazole ( oflox TZ ). As per ICH guidelines the results of the analysis were validated statistically and were found to be satisfactory.
KEYWORDS:
Bromophenol blue, ion pair complex, spectrophotometry, validation.
Cite:
Y. Haribabu, Sheeja Velayudhan Kutty, Vidhya P.M., Greeshma S. Extractive Spectrophotometric Determination of Ofloxacin and Tinidazole with Bromophenol Blue. Research J. Pharm. and Tech 6(7): July 2013; Page 726-730.
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