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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   6

Issue No. :  4

Year :  2013

Pages :   435-439

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Direct shoots regeneration from explant surface in Euphorbia hirta L.

Address:   M.S. Shekhawat*
Biotechnology Unit, K.M. Centre for Postgraduate Studies, Pondicherry, India
*Corresponding Author
DOI No: Not Available

A highly efficient plant propagation protocol was developed for Euphorbia hirta L. using nodal shoot segments as explant. The explants were surface sterilized with 0.1% (w/v) mercuric chloride on Murashige and Skoog (MS) medium augmented with different concentrations of 6-Benzylaminopurine (BAP) and Kinetin (Kn) singly or in combination with Indole-3-acetic acid (IAA). Use of BAP at 2.0 mgl-1 induced the highest frequency (100%) of shoot induction as well as maximum number of shoots per explant (6.22). Proliferated shoot clumps were further multiplied and elongated on 0.5 mgl-1 BAP and Kn. The highest rooting frequency (92.8%) as well as highest number of roots (4.3) was observed in half strength MS medium supplemented with 2.0 mgl-1 Indole-3-butyric acid (IBA). Regenerated plantlets were acclimatized successfully in the green house and finally transplanted in the pots.
Euphorbia hirta, in vitro, rooting, green house.
M.S. Shekhawat. Direct shoots regeneration from explant surface in Euphorbia hirta L. Research J. Pharm. and Tech. 6(4): April 2013; Page 435-439.
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