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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   6

Issue No. :  4

Year :  2013

Pages :   435-439

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Direct shoots regeneration from explant surface in Euphorbia hirta L.



Address:   M.S. Shekhawat*
Biotechnology Unit, K.M. Centre for Postgraduate Studies, Pondicherry, India
*Corresponding Author
DOI No: Not Available

ABSTRACT:
A highly efficient plant propagation protocol was developed for Euphorbia hirta L. using nodal shoot segments as explant. The explants were surface sterilized with 0.1% (w/v) mercuric chloride on Murashige and Skoog (MS) medium augmented with different concentrations of 6-Benzylaminopurine (BAP) and Kinetin (Kn) singly or in combination with Indole-3-acetic acid (IAA). Use of BAP at 2.0 mgl-1 induced the highest frequency (100%) of shoot induction as well as maximum number of shoots per explant (6.22). Proliferated shoot clumps were further multiplied and elongated on 0.5 mgl-1 BAP and Kn. The highest rooting frequency (92.8%) as well as highest number of roots (4.3) was observed in half strength MS medium supplemented with 2.0 mgl-1 Indole-3-butyric acid (IBA). Regenerated plantlets were acclimatized successfully in the green house and finally transplanted in the pots.
KEYWORDS:
Euphorbia hirta, in vitro, rooting, green house.
Cite:
M.S. Shekhawat. Direct shoots regeneration from explant surface in Euphorbia hirta L. Research J. Pharm. and Tech. 6(4): April 2013; Page 435-439.
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