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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   6

Issue No. :  3

Year :  2013

Pages :   260-263

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Development and Validation of a UV- Spectrophotometric Method for Simultaneous Estimation of Hydrochlorothiazide and Losartan Potassium in Bulk Drug and Its Formulations



Address:   Babu Lal Saini1*, Pramod Kumar1, Ravi Dahiya1, Javed Akhtar2, Birendra Shrivastav1
1Department of Quality Assurance, School of Pharmaceutical Sciences, Jaipur National University, Jagatpura, Jaipur (Rajasthan) India-302025
2Department of Quality Assurance, Parul Institute of Pharmacy & Research, Baroda
*Corresponding Author
DOI No: Not Available

ABSTRACT:
A simple, efficient, precise and accurate Vierodt’s method have been developed for the estimation of Hydrochlorothiazide and Losartan potassium in pure and in fixed dose Combination. In this method UV spectrum of Hydrochlorothiazide and Losartan potassium were overlained which involves the formation of Simultaneous equation at maximum wavelength 274 nm and 234 nm. Both the drugs obeyed Beer’s law in the concentration range 5-20 microgram/ml and 1-10 microgram/ml for Hydrochlorothiazide and Losartan potassium, respectively. The accuracy of the method was determined by recovery studies and was found to be in the range of 102.29-103.10% and 99.52-101.60% for Hydrochlorothiazide and Losartan potassium, respectively. The method was validated as per ICH guidelines and statically. The method showed good reproducibility and recovery with % RSD less than 2. The method was found to be simple, economic, accurate and reproducible and can be used for routine analysis of Hydrochlorothiazide and Losartan potassium in pure and in fixed dose combination.
KEYWORDS:
Simultaneous equation method, Vierodt’s method, Hydrochlorothiazide, Losartan potassium, ICH guidelines, method validation.
Cite:
Babu Lal Saini, Pramod Kumar, Ravi Dahiya, Javed Akhtar, Birendra Shrivastav. Development and Validation of a UV- Spectrophotometric Method for Simultaneous Estimation of Hydrochlorothiazide and Losartan Potassium in Bulk Drug and Its Formulations. Research J. Pharm. and Tech. 6(3): March 2013; Page 260-263.
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