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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   6

Issue No. :  3

Year :  2013

Pages :   251-254

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Evaluation of membrane stabilizing and inhibition of protein denaturation activity of Phyllanthus fraternus Webster



Address:   Atul Ramchandra Chopade* and Fahim Jehangir Sayyad
Dept. of Pharmacology and Pharmacognosy, Government College of Pharmacy, Karad, District,- Satara. 415124, Maharashtra, India.
*Corresponding Author
DOI No: Not Available

ABSTRACT:
Objective: To evaluate the membrane stabilizing potential and inhibition of protein denaturation property of hydroalcoholic extract Phyllanthus fraternus Webster [PF]. Method – Hydroalcoholic extract of PF was evaluated using the human red blood cells (HRBC) membrane stabilization method as HRBC membranes are similar to lysosomal membrane components. The prevention of hypotonicity-induced HRBC membrane lysis was taken as a measure of anti-inflammatory activity of PF extract. While the extract was also incubated with egg albumin and subjected to determination of absorbance and viscosity to assess the anti-inflammatory property. Results- The hydroalcoholic extract of PF in concentration of 25-800 microgram/ml showed a concentration dependent inhibition of haemolysis of erythrocytes induced by hypotonic solution. The hydroalcoholic extract of PF also showed a concentration dependent inhibition of protein (albumin) denaturation with IC50 of 52 microgramml. Conclusion- From the study it can be postulated that the anti-inflammatory activity of Phyllanthus fraternus Webster could be due to its membrane stabilizing action and inhibition of protein denaturation.
KEYWORDS:
Phyllanthus fraternus Webster, anti-inflammatory, membrane stabilizing & protein denaturation
Cite:
Atul Ramchandra Chopade, Fahim Jehangir Sayyad. Evaluation of membrane stabilizing and inhibition of protein denaturation activity of Phyllanthus fraternus Webster. Research J. Pharm. and Tech. 6(3): March 2013; Page 251-254.
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