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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   6

Issue No. :  2

Year :  2013

Pages :   191-194

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Simultaneous Estimation of Tenofovir Disoproxil Fumarate, Efavirenz and Lamivudine in Bulk and Tablet Dosage Form by UV Spectrophotometry



Address:   Murugan S.*, Subhashis Debnath, Pranabesh Sikdar, Niranjan Babu M.
Seven Hills College of Pharmacy, Tirupati-517 561, Andhra Pradesh, India
*Corresponding Author
DOI No: Not Available

ABSTRACT:
A simple, sensitive, rapid spectrophotometric method have been developed for simultaneous estimation of Tenofovir disoproxil fumarate (TDF), Efavirenz (EFV) and Lamivudine (LAM) in bulk and in tablet dosage form.The simultaneous equations based on measurement of absorbance at three wavelengths 261 nm, 267 nm and 271 nm Lambda max of TDF, EFV and LAM respectively. Beer’s law was obeyed in the concentration range of 2-12 microgram/ml, 3-18 microgram/ml and 2-12 microgram/ml for TDF, EFV and LAM. The methods were validated as per ICH guidelines. Statistical analysis proved that the methods were accurate, precise and reproducible for analysis of LAM, TDF and EFV in tablet dosage form. The wide linearity range, sensitivity, accuracy and simple procedure imply that the proposed technique demonstrated to be appropriate for routine analysis and quality control assay of tablet.
KEYWORDS:
Tenofovir disoproxil fumarate, Efavirenz, Lamivudine, Simultaneous equation method.
Cite:
Murugan S., Subhashis Debnath, Pranabesh Sikdar, Niranjan Babu M. Simultaneous Estimation of Tenofovir Disoproxil Fumarate, Efavirenz and Lamivudine in Bulk and Tablet Dosage Form by UV Spectrophotometry. Research J. Pharm. and Tech. 6(2): Feb. 2013; Page 191-194.
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