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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   6

Issue No. :  2

Year :  2013

Pages :   178-183

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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RP-HPLC Method Development and Validation for the Simultaneous Estimation of Ibuprofen and Methocarbamol in Ibuprofen-Methocarbamol Caplets



Address:   Nataraj K.S.1*, Ravinder Reddy S.2, Kiran Kumar D.3 and Kesinath Reddy K.4
1Shri Vishnu College of Pharmacy, Bhimavaram – 534202, West Godavari District, Andhra Pradesh.
2University College of Technology, Osmania University, Hyderabad.
3Center for Pharmaceutical Sciences, IST, JNTUH, Hyderabad.
4St. Peters Institute of Pharmaceutical Sciences, Hanamkonda, Warangal-506001
*Corresponding Author
DOI No: Not Available

ABSTRACT:
A reverse phase high performance liquid chromatography (RP-HPLC) has been developed and validated as a stability indicating assay of Ibuprofen (IBP) and Methocarbamol (MCB). The degradants formed were well separated from the main peaks. The separation was achieved by isocratic elution on Inertsil ODS-3V Column (150 mm x 4.6mm x 5 µm) and 0.1M Phosphate buffer pH 3.5: methanol: acetonitrile and tetrahydrofuran (20:25:55:0.3) used as a mobile phase at a flow rate of 1ml/min. The detection was carried out at 220nm. Retention time of Ibuprofen and Methocarbamol were found to be 8.6 and 3.7 min respectively. The method has been validated for linearity, accuracy and precision. Linearity for Ibuprofen and Methocarbamol were in the range of 25%-175% concentration of standard preparation. The mean recoveries obtained for Ibuprofen and Methocarbamol were found to be 100.61% and 99.23% respectively. Developed RP-HPLC method was found to be accurate, precise, selective and rapid for simultaneous estimation of Ibuprofen and Methocarbamol in tablets.
KEYWORDS:
RP-HPLC; Ibuprofen; Methocarbamol and Recovery.
Cite:
Nataraj K.S., Ravinder Reddy S., Kiran Kumar D., Kesinath Reddy K. RP-HPLC Method Development and Validation for the Simultaneous Estimation of Ibuprofen and Methocarbamol in Ibuprofen-Methocarbamol Caplets. Research J. Pharm. and Tech. 6(2): Feb. 2013; Page 178-183.
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