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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   6

Issue No. :  10

Year :  2013

Pages :   1107-1110

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Development and Validation of RP-HPLC Method for Simultaneous Estimation of Cholecalcererol (Vitamin D3) and Tocopherol Acetate (Vitamin E) in Powder Dosage Form



Address:   Thorat Shashikant D.*, Ghodeswar B. C., Darode G. S., Shinde N. S., Chaudhari S. R.
Department Pharmaceutical Chemistry, Amrutvahini Collage of Pharmacy, Sangamner, Dist- Ahmednagar, Maharashtra, India.
*Corresponding Author
DOI No: Not Available

ABSTRACT:
A simple, specific, accurate and precise Reverse Phase High Performance Liquid Chromatography method was developed for simultaneous estimation of Cholecalceferol (Vitamin D3) and Tocopherol Acetate (Vitamin E) in powder dosage form on RP C-18 Column (EC 250/4 neucleosil 100-10) using Methanol : Water (97:3 %v/v) as mobile phase. The flow rate was 1.0 ml/min and effluent was monitored at 274nm. The retention time for Cholecalceferol (Vitamin D3) and Tocopherol Acetate (Vitamin E) was found to be as 8.78 and 12.80 respectively. Proposed method was validated for Specificity, Precision, Accuracy, Linearity, and Robustness.
KEYWORDS:
Cholecalceferol, Tocopherol Acetate, Reverse Phase High Performance Liquid Chromatography.
Cite:
Thorat Shashikant D., Ghodeswar B. C., Darode G. S., Shinde N. S., Chaudhari S. R. Development and Validation of RP-HPLC Method for Simultaneous Estimation of Cholecalcererol (Vitamin D3) and Tocopherol Acetate (Vitamin E) in Powder Dosage Form. Research J. Pharm. and Tech. 6(10): October 2013; Page 1107-1110.
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