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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   5

Issue No. :  7

Year :  2012

Pages :   938-941

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Development of RP-HPLC Method for Simultaneous Estimation of Alprazolam and Fluoxetine Hydrochloride in Pharmaceutical Tablet Dosage Form



Address:   S. K. Shah1*, S. C. Dangre2, N. B. Charbe2
1J. L. Chaturvedi College of Pharmacy, Electronic Zone Building, MIDC, Hingna road, Nagpur, Maharashtra, India.
2Shrarad Pawar College of Pharmacy, Hingna Road, Wanadongri, Nagpur-440016, Maharashtra, India.
*Corresponding Author
DOI No: Not Available

ABSTRACT:
A new, simple, accurate and precise reversed phase high performance chromatographic method with UV detection was developed for the simultaneous quantitative estimation of Alprazolam (APZ) and Fluoxetine hydrochloride (FLX) in combined tablet dosage form. The method employed on reversed phase liquid chromatographic analysis using C18 column (4.6 × 250 mm, 5 µm). The Eluent was monitored by absorbance at wavelength 225 nm using a mixture of Acetonitrile and phosphate buffer (50:50 v/v) at pH 4.5 with a flow rate of 1.0 mL min-1. Calibration curves were found to be linear in the concentration range of 0.5-2.00 µg mL-1 and 40-160 µg mL-1 for APZ and FLX respectively. The proposed method was validated by testing its Linearity, recovery, repeatability and it was successfully employed for the rapid and specific simultaneous quantitative estimation of APZ and FLX in combined tablet dosage form
KEYWORDS:
Alprazolam, Fluoxetine Hydrochloride, RP-HPLC, Method development, Validation, Marketed formulation.
Cite:
S. K. Shah, S. C. Dangre, N. B. Charbe. Development of RP-HPLC Method for Simultaneous Estimation of Alprazolam and Fluoxetine Hydrochloride in Pharmaceutical Tablet Dosage Form. Research J. Pharm. and Tech. 5(7): July 2012; Page 938-941.
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