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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   5

Issue No. :  6

Year :  2012

Pages :   785-788

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Isolation and Detection of Aspergillus and Candida Species in Sputum of HIV Positive Patients and It Correlate With CD4



Address:   Neetu Jangir1*, Saroj Bakshi1 and Nitya Vyas2
1Department of Zoology, University of Rajasthan, Jaipur (Rajasthan).
2Department of Microbiology, S.M.S Medical College, Jaipur (Rajasthan).
*Corresponding Author
DOI No: Not Available

ABSTRACT:
Aspergillus and Candida causing important role in oppurinstic infection was an infection caused by pathogens that usually do not cause disease in a healthy immune system. 50 patients were screened for HIV at ICTC , SMS Medical college. Aspergillus and Candida were identified on the basis of culture and microscopic examination as Gram’s and lactophenol cotton blue staining. Aspergillus is a filamentous, cosmopolitian and ubiquitous fungus whereas Candida is a yeast. Both species were endosymbionts of animal hosts including human. Clinically, Candida albicans was the most significant sub-species and causing Candidiasis infection in human and other animals, especially in immune compromised patients. ELISA and HIV comb test were to evaluate the presence of antigen or antibody in a sample. On SDA (Sabouraud Dextrose Agar), samples clinically inoculated in media for recovery, sub-culture and identification of fungi. Gentamicin acts as antibacterial agent whereas Cyclohexamide inhibits the growth. The results evaluate that grayish-green colonies of Aspergillus and white-creamy colonies of Candida on SDA. Results of investigation that, out of 50 cases, 42 people infected by Candida and 8 people with Aspergillus. Males were more infected to HIV. Normal CD4 counts in adult ranges from 500 to 1500 cells per cubic millimetre of blood. In general, the CD4 count goes down as HIV diseases progresses. Maximum fungus growth in between 30-40 years. The examination also evaluate that the HIV infected individuals belong to sexually active age group between 20-40 years. Sexual contact was the main mode of transmission which was more prevalence in males.
KEYWORDS:
Candida albicans ,Sabouraud Dextrose Agar, CD4 count, HIV, Immunocompromised patients, Cyclohexamide.
Cite:
Neetu Jangir, Saroj Bakshi, Nitya Vyas. Isolation and Detection of Aspergillus and Candida Species in Sputum of HIV Positive Patients and It Correlate With CD4. Research J. Pharm. and Tech. 5(6): June 2012; Page 785-788.
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