2nd International Conference on Fostering Interdisciplinary Research In Health Sciences (ICFIRHS) 2019 (01-May-2019)        |

Journal :   Research Journal of Pharmacy and Technology

Volume No. :   5

Issue No. :  6

Year :  2012

Pages :   829-833

ISSN Print :  0974-3618

ISSN Online :  0974-360X


Registration

Allready Registrered
Click to Login

Development and validation of HPLC method for the determination of pregabalin in bulk and in pharmaceutical formulations



Address:   Prashant Pingale *, Tanmay Singasane
SPTM, SVKM’s NMIMS, Shirpur, Dhule-425 405, Maharashtra, India
*Corresponding Author
DOI No: Not Available

ABSTRACT:
Pregabalin, a a-amino-n-butyric acid derivative, is an antiepileptic drug which is not yet official in any pharmacopoeia. Thus, the development of analytical procedures for this drug in bulk/ formulation form is need of the day. We, herein, report a new, simple, specific, accurate, cost effective and reproducible spectrophotometric method for the determination of pregabalin. The chromatography was set on Kromasil, C18, 100 x 4.6mm, 5 µm column using UV detector. The mobile phase consitituted of phosphate buffer pH 6.9 and acetonitrile in the ratio of 90:10 with the flow rate of 1ml/min. The method was validated as per ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness. Also, the developed method was successfully applied to determination of pregabalin in pharmaceutical formulations without any interference from common excipients. Hence, this method can be very useful for routine laboratory analysis of pregabalin.
KEYWORDS:
Method development, Pregabalin, RP-HPLC, Validation, Simple.
Cite:
Prashant Pingale, Tanmay Singasane. Development and validation of HPLC method for the determination of pregabalin in bulk and in pharmaceutical formulations. Research J. Pharm. and Tech. 5(6): June 2012; Page 829-833.
[View HTML]      [View PDF]



Visitor's No. :   641004