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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   5

Issue No. :  4

Year :  2012

Pages :   533-537

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Comparative Pharmacognostical characters of the dried seeds and mesocarp of Benincasa hispida



Address:   Hayat M. Mukhtar*, Deepika Vashishth, Babar Ali, Rupinder Kaur
Natural Product Research Laboratory, Department of Pharmacognosy and Phytochemistry, Shaheed Bhagat Singh College of Pharmacy, Patti, Amritsar, Punjab- 143416
*Corresponding Author
DOI No: Not Available

ABSTRACT:
This specification covers the standardization and preliminary phytochemical investigation of the plant Benincasa hispida (Cucurbitaceae). It is widely used as antiulcer, anti-angiogenic, bronchodilator, in opioid withdrawal benefit, anti-diabetic and in mental disability. The plant material was subjected to pharmacognostical studies like macroscopic and microscopic studies (powder microscopy, micrometry, histochemical tests and transverse section). The microscopic characters revealed the presence of: Seeds [lignified trichomes found in groups (17.28µ,1.62µ), transparent fibers (70.20µ, 1.35µ), round shaped oil cells (9.45µ) found in thin walled parenchyma cells, parenchyma cells with wrinkled walls and lignified spiral vessels (52.38µ, 1.48µ)] mesocarp [lignified covering trichomes (27.43µ, 1.73µ) fibers (34.10µ, 2.22µ), lignified parenchyma cells (14.58) and vascular bundles]. Dried plant material was subjected to various physiochemical parameters like Hot extraction- seeds [water (15%) alcohol (7%)] mesocarp [water (60%) alcohol (25%)], ash values {seeds [total ash value (4%), water soluble ash (1%), acid insoluble ash (0.5%) sulphated ash value (3%)] mesocarp [total ash value (10%), water soluble ash (8%), acid insoluble ash (0.5%) sulphated ash value (7%)]}, cold maceration- {[petroleum ether [seeds (29%) mesocarp (1%)], benzene [seeds (28%) mesocarp (2%)], ethyl acetate [seeds (28%) mesocarp (2%)], chloroform [seeds (28%) mesocarp (2%)], acetone [seeds (30%) mesocarp (4%)], methanol [seeds (12%) mesocarp (31%)], water [seeds (11%) mesocarp (44%)]}, TLC with different solvents and at different wavelengths, fluorescent analysis, loss on drying, foaming index, swelling index, microscopic studies (powder microscopy, micrometry, transverse section, histo-chemical tests), successive extraction {[petroleum ether [seeds (18%) mesocarp (1.2%)], benzene [seeds (1.2%) mesocarp (1.2%)], ethyl acetate [seeds (1.8%) mesocarp (3.6%)], chloroform [seeds (1.6%) mesocarp (5.6%)], acetone [seeds (2.4%) mesocarp (8.8%)], methanol[seeds (4%) mesocarp (15.2%)]. The preliminary phytochemical investigation revealed the presence of amino acids, triterpenoids, tannins, carbohydrates. These parameters can be utilized for quick identification of the Benincasa hispida and are particularly useful in powdered form.
KEYWORDS:
Benincasa hispida, solvent extracts, UV chamber, amino acids, triterpenoids.
Cite:
Hayat M. Mukhtar, Deepika Vashishth, Babar Ali, Rupinder Kaur. Comparative Pharmacognostical characters of the dried seeds and mesocarp of Benincasa hispida. Research J. Pharm. and Tech. 5(4): April 2012; Page 533-537.
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