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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   5

Issue No. :  12

Year :  2012

Pages :   1573-1576

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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A RP-HPLC Method Development and Validation for the Estimation of Tadalafil in Bulk and Pharmaceutical Dosage Forms



Address:   Saroj Kumar Raul1*, B.V.V Ravi kumar2, Ajaya Kumar Pattnaik3
1M. R. College of Pharmacy, Phool-Baugh, Vizianagaram, A.P, India.
2Roland Institute of Pharmaceutical Sciences, Berhampur, Orissa, India.
3Departments of Chemistry, Ravenshaw University, Cuttack, Orissa, India.
*Corresponding Author
DOI No: Not Available

ABSTRACT:
A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of Tadalafil in pharmaceutical dosage form. Isocratic elution at a flow rate of 1.0 mL min -1 was employed on a Symmetry C18 column at ambient temperature. The mobile phase consisted of acetonitrile: buffer 50:50 (v/v) and the detection wavelength was at 284 nm. Linearity was observed in concentration range of 20-70 µg/mL. The retention time for Tadalafil was 2.98 min. The method was validated as per the ICH guidelines. The proposed method can be successfully applied for the estimation of Tadalafil in pharmaceutical dosage forms.
KEYWORDS:
Estimation, Method development, Validation, Tadalafil, RP-HPLC.
Cite:
Saroj Kumar Raul, B.V.V Ravi kumar, Ajaya Kumar Pattnaik. A RP-HPLC Method Development and Validation for the Estimation of Tadalafil in Bulk and Pharmaceutical Dosage Forms. Research J. Pharm. and Tech. 5(12): Dec. 2012; Page 1573-1576.
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