List of Refereed Journals published by UGC, New Delhi on 28-03-2017-- AJRC (Sr. No.-1025); AJM (Sr. No.-2497); RJET (Sr. No.-7220); RJPP (Sr. No.- 7232): RJPPD (Sr. No.- 7234); RJPT (Sr. No.-7235) (19-Apr-2017)        | List of Refereed Journals published by UGC, New Delhi on 11-01-2017-- Research Journal of Pharmacy and Technology (Sr. No.-275); Asian Journal of Management (Sr. No.-3986); Asian Journal of Research in Chemistry (Sr. No.-4008); Research Journal of Engineering and Technology (Sr. No.-31787); Research (22-Jan-2017)        |

Journal :   Research Journal of Pharmacy and Technology

Volume No. :   4

Issue No. :  7

Year :  2011

Pages :   1078-1082

ISSN Print :  0974-3618

ISSN Online :  0974-360X


Registration

Allready Registrered
Click to Login

Simultaneous Determination of Cefixime Trihydrate and Erdosteine in Human Plasma by HPTLC Method



Address:   Madgulkar Ashwini R., Sonawane Prajakta A., Dhoka Madhura V.* and Nimbalkar Umesh A.
Department of Quality Assurance, AISSMS College of Pharmacy, Kennedy Road, Near RTO, Pune- 41100, India
*Corresponding Author
DOI No: Not Available

ABSTRACT:
A simple, sensitive high performance thin layer chromatographic method was developed for simultaneous determination of Cefixime Trihydrate and Erdosteine in human plasma. The plasma samples were subjected to the protein precipitation by using acetonitrile. The separation was achieved using dichloromethane: methanol: triethylamine (6.5:3.5:0.07v/v/v) as mobile phase and silica gel Plate 60F254 as stationary phase. Densitometric analysis was carried out at wavelength 235nm. The Rf values for Cefixime Trihydrate and Erdosteine was found to be 0.55±0.03 and 0.75±0.03 respectively. The calibration curves were linear over range of 100-600 ng/spot for Cefixime Trihydrate and 150-750 ng/spot for Erdosteine. The average recovery of Cefixime Trihydrate and Erdosteine was found to be 97.6 % and 97.5 % respectively. Method was found to be reproducible with % coefficient of variance (%CV) for intra and interday precision of not more than 15 for QC samples and not more than 20 for LLOQ over the said concentration range.
KEYWORDS:
Cefixime Trihydrate (Cef), Erdosteine (ERDO), Human plasma, Densitometry, HPTLC.
Cite:
Madgulkar Ashwini R., Sonawane Prajakta A., Dhoka Madhura V., Nimbalkar Umesh A. Simultaneous Determination of Cefixime Trihydrate and Erdosteine in Human Plasma by HPTLC Method. Research J. Pharm. and Tech. 4(7): July 2011; Page 1078-1082.
[View HTML]      [View PDF]



Visitor's No. :   264751