Journal :   Research Journal of Pharmacy and Technology

Volume No. :   4

Issue No. :  6

Year :  2011

Pages :   982-986

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Effect of Gamma Irradiation on Microbial Load of Kalmegh

Address:   Mamatha A.1* and Kalpana S. Patil2
1Dept. of Pharmacognosy, KLE University’s College of Pharmacy, Rajajinagar II Block, Bangalore 560 010.
2Department of Pharmacognosy, KLE University’s College of Pharmacy, Nehru Nagar, Belgaum 590 010.
*Corresponding Author
DOI No: Not Available

Whole plant powder of Kalmegh (Andrographis paniculata) was exposed to gamma irradiation at doses of 5 and 10 kGy in a Co 60 irradiator. Irradiated and unirradiated samples were stored at room temperature. Microbial load of whole plant powder were evaluated at 0, 6 and 12 months of storage. Results indicated that gamma irradiation reduced the total aerobic count and total fungal count in comparison to the non-irradiated sample. Further, the non-irradiated samples showed the presence of E.coli and Staphylococcus aureus. On irradiation there was a drastic reduction in E.coli and Staphylococcus aureus. Microbial load was found to be increased after 6 and 12 months of storage in the non-irradiated samples, where as there were no significant differences in the irradiated samples. A dose of 5 kGy could reduce the microbial load to acceptable levels, where as commercial sterility could be obtained at a dose of 10kGy. Thus, gamma irradiation can be used as a suitable technique for microbial decontamination of phytopreparations and products of Kalmegh and thus increase their shelf life and stability.
Kalmegh, Gamma irradiation, microbial load, shelf life, stability, microbial decontamination.
Mamatha A., Kalpana S. Patil. Effect of Gamma Irradiation on Microbial Load of Kalmegh. Research J. Pharm. and Tech. 4(6): June 2011; Page 982-986.
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