List of Refereed Journals published by UGC, New Delhi on 28-03-2017-- AJRC (Sr. No.-1025); AJM (Sr. No.-2497); RJET (Sr. No.-7220); RJPP (Sr. No.- 7232): RJPPD (Sr. No.- 7234); RJPT (Sr. No.-7235) (19-Apr-2017)        | List of Refereed Journals published by UGC, New Delhi on 11-01-2017-- Research Journal of Pharmacy and Technology (Sr. No.-275); Asian Journal of Management (Sr. No.-3986); Asian Journal of Research in Chemistry (Sr. No.-4008); Research Journal of Engineering and Technology (Sr. No.-31787); Research (22-Jan-2017)        |

Journal :   Research Journal of Pharmacy and Technology

Volume No. :   4

Issue No. :  6

Year :  2011

Pages :   951-954

ISSN Print :  0974-3618

ISSN Online :  0974-360X


Registration

Allready Registrered
Click to Login

Development and Validation of Spectrophotometric Methods for the estimation of Gemcitabine in Bulk Drug and its Pharmaceutical Formulations.



Address:   Prakash S. Sarsambi1*, Kalyan K.1, S.A. Raju1 and L.S. Danki1
1Department of Pharmaceutical Analysis, HKES’s College of pharmacy, Sedam road, Gulbarga, Karnataka-585105, India.
*Corresponding Author
DOI No: Not Available

ABSTRACT:
Three simple, precise and sensitive visible spectrophotometric methods (A, B and C) have been developed and validated for the quantitative estimation of Gemcitabine in bulk drug and its pharmaceutical formulations. Method A is based on Diazotization followed by coupling of Bratton Marshall Reagent (BMR) with Gemcitabine to form red colored chromogen, which exhibits absorption maxima at 554nm and obeys Beer’s law in the concentration range of 50-250µg/ml. Method B and C is based on ion pair formation between the drug and two acidic dyes, namely Bromocresol green(BCG) and Bromocresol purple (BCP), which were measured at 418 and 413nm respectively and Beer’s law obey’s in the concentration range of 20-100µg/ml and 50-250µg/ml respectively. The results of the proposed methods were validated statistically, recovery studies and are compared with those obtained by the UV method. The proposed methods were applied successfully to the determination of gemcitabine in commercial dosage forms.
KEYWORDS:
Gemcitabine, Visible spectroscopy, BMR, BCG and BCP.
Cite:
Prakash S. Sarsambi, Kalyan K., S.A. Raju, L.S. Danki. Development and Validation of Spectrophotometric Methods for the estimation of Gemcitabine in Bulk Drug and its Pharmaceutical Formulations. Research J. Pharm. and Tech. 4(6): June 2011; Page 951-954.
[View HTML]      [View PDF]



Visitor's No. :   175054