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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   4

Issue No. :  5

Year :  2011

Pages :   759-761

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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High Performance Liquid Chromatographic Estimation of Prulifloxacin in Pharmaceutical Dosage Form



Address:   M. Padmalatha1, Syeda Kulsum1, K. Vanitha Prakash2*.
1Department of Pharmaceutical Analysis, Vijaya College of Pharmacy, Hyderabad, A.P India.
2SSJ College of Pharmacy, V. N. Pally, Gandipet, Hyderabad, A.P. India.
*Corresponding Author
DOI No: Not Available

ABSTRACT:
A simple reverse phase liquid chromatographic method was developed and validated as per the ICH guidelines for the quantitative determination of Prulifloxacin in pharmaceutical dosage forms. The method was simple, precise, specific and accurate. The mobile phase consists of 0.2% acetic acid in water, acetonitrile and methanol in the ratio (7:2:1). The diluent used was 0.1% acetic acid in water: acetonitrile (1:9). The proposed RP-HPLC method utilizes an Inertsil ODS-3v, C18 column (250mm ×4.6mm) at ambient temperature. The eluent was monitored at 273nm and retention time of Prulifloxacin was 10.46 min. The linearity was observed from 12.5-75_g/ml with r2= 1. The limit of detection and limit of quantitation were found to be 0.75_g/ml and 1.5_g/ml respectively. The method was demonstrated to be precise, accurate and specific. The results indicated that the proposed method could be used in the assay of pharmaceutical dosage form of prulifloxacin.
KEYWORDS:
Prulifloxacin, RP- HPLC, Validation, acetonitrile.
Cite:
M. Padmalatha, Syeda Kulsum, K. Vanitha Prakash. High Performance Liquid Chromatographic Estimation of Prulifloxacin in Pharmaceutical Dosage Form. Research J. Pharm. and Tech. 4(5): May 2011; Page 759-761.
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