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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   4

Issue No. :  4

Year :  2011

Pages :   638-641

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Simultaneous RP-HPLC Determination of Diclofenac Potassium and Famotidine in Pharmaceutical Preparations



Address:   R.V Rele*, J.M. Parab, V.V. Mhatre and C.B. Warkar D. G. Ruparel College, Matunga, Mumbai 400 016. *Corresponding Author
DOI No: Not Available

ABSTRACT:
A simple, fast and precise reverse phase high performance liquid chromatography method is developed for the simultaneous determination of famotidine and diclofenac potassium using methyl paraben as an internal standard. Chromatographic separation of these two drugs was performed on Inertsil C18 column ( 250 mm x 4.6 mm, 5 µm ) as stationary phase with a mobile phase comprising of 0.05 M KH2PO4 : methanol (35:65 v/v) at a flow rate of 1.5ml / min and UV detection at 254 nm. The retention time of famotidine, methyl paraben and diclofenac potassium were 1.95 min, 3.57 min and 12.66 min respectively. The proposed method was validated for linearity, accuracy, precision, limit of detection, limit of quantitation and solution stability. Linearity, accuracy and precision were found to be acceptable over the ranges of 100 – 300 µg / ml for famotidine and 250 – 750 µg / ml for diclofenac potassium. This method can be conveniently adopted for routine quality control analysis.
KEYWORDS:
Famotidine, Diclofenac potassium, RP-HPLC
Cite:
R.V Rele, J.M. Parab, V.V. Mhatre, C.B. Warkar. Simultaneous RP-HPLC Determination of Diclofenac Potassium and Famotidine in Pharmaceutical Preparations. Research J. Pharm. and Tech. 4(4): April 2011; Page 638-641.
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