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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   4

Issue No. :  4

Year :  2011

Pages :   582-584

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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High Performance Thin Layer Chromatographic Determination of Ethinylestradiol and Norgestimate in Combined Tablet Dosage Form



Address:   P.B. Deshpande*, S.V. Gandhi, A.V. Patel, V.A. Khandelwal, U.V. Bhavsar and B.P. Pawaskar
Dept. of Pharmaceutical Analysis, AISSMS College of Pharmacy, Kennedy Road, Near R.T.O., Pune – 411 001
*Corresponding Author
DOI No: Not Available

ABSTRACT:
A new simple High Performance Thin Layer Chromatographic (HPTLC) method for determination of Ethinylestradiol (EE) and Norgestimate (NGM) in combined tablet dosage form has been developed and validated. The mobile phase selected was Benzene: Ethyl acetate: Triethylamine (7: 2: 1 v/v/v) with UV detection at 280 nm. The retention factors for EE and NGM were found to be 0.62 ± 0.102 and 0.82 ± 0.123. The method was validated with respect to linearity, accuracy, precision and robustness. Results found to be linear in the concentration range of 200-1000 ng/band for EE and 1000-5000 ng/band for NGM. The method has been successfully applied for the analysis of drugs in pharmaceutical formulation. The % assay (Mean ± S.D.) was found to be 99.4 % ± 0.157 for EE and 99.8 % ± 0.14 for NGM.
KEYWORDS:
Ethinylestradiol, Norgestimate, High Performance Thin Layer chromatography.
Cite:
P.B. Deshpande, S.V. Gandhi, A.V. Patel, V.A. Khandelwal, U.V. Bhavsar, B.P. Pawaskar. High Performance Thin Layer Chromatographic Determination of Ethinylestradiol and Norgestimate in Combined Tablet Dosage Form. Research J. Pharm. and Tech. 4(4): April 2011; Page 582-584.
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