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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   4

Issue No. :  3

Year :  2011

Pages :   437-440

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Visible Spectrophotometric Estimation of Emtricitabine in Pharmaceutical Formulations



Address:   P. Janaki Pathi1*, P. Raveendra Reddy2 and N. Appala Raju3
1Analytical Department, Vishnu Chemicals Limited, Jeedimetla, Hyderabad. 1,2Department of Chemistry, Sri Krishnadevaraya University, Anantapur-515 003
3Department of Pharmaceutical Chemistry, Sultan-Ul-Uloom College of Pharmacy Mount Pleasant, Road # 3, Banjara Hills, Hyderabad-500 034
.*Corresponding Author
DOI No: Not Available

ABSTRACT:
Three simple, accurate, rapid and sensitive methods (A, B and C) have been developed for the estimation of Emtricitabine in its pharmaceutical dosage form. The Method A is based on the formation of orange red colored chromogen, due to reaction of Emtricitabine with p-Dimethyl amino Cinnamaldehyde (PDAC) reagent in the presence of methanol, which exhibits ? max at 538 nm. Method B is based on the reaction of Emtricitabine with 3-methyl-2-benzothiazolinone hydrazone (MBTH) in the presence of Cerric ammonium sulphate to form a bluish green colored chromogen, which shows maximum absorbance at 635 nm. The Method C is based on the formation of reddish brown colored chromogen with Mordant Black Dye in the presence of Phthalate buffer, which shows absorption maximum at 543 nm. The absorbance-concentration plot is linear over the range of 1-10 µg/mL for Method A, 2-18 µg/mL for Method B and 10-90 µg/mL for Method C. Results of analysis for all the methods were validated statistically and by recovery studies. Literature survey reveals only HPLC methods deals with biological fluids and in fixed pharmaceutical dosage forms. The proposed methods are economical and sensitive for the estimation of Emtricitabine in bulk drug and in its formulations.
KEYWORDS:
UV-Visible Spectrophotometry, Emtricitabine, Ferric chloride, p-dimethylaminocinnamaldehyde (PDAC), 3-methyl 2-benzothiazolinone hydrazone,(MBTH), Mordant Black III, Potassium Phthalate.
Cite:
P Janaki Pathi, P Raveendra Reddy, N Appala Raju. Visible Spectrophotometric Estimation of Emtricitabine in Pharmaceutical Formulations. Research J. Pharm. and Tech. 4(3): March 2011; Page 437-440.
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