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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   4

Issue No. :  2

Year :  2011

Pages :   242-246

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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A Sensitive UV Spectrophotometric Analytical Method Development, Validation and Preformulation Studies of Clarithromycin



Address:   Putta Rajesh Kumar*, Somashekar Shyale, M. Mallikarjuna Gouda and S.M. Shanta Kumar
Department of Pharmaceutics and Pharmaceutical Chemistry, V.L.College of Pharmacy, Raichur – 584103, Karnataka, India.
*Corresponding Author
DOI No: Not Available

ABSTRACT:
Clarithromycin is a broad spectrum macrolide antibacterial agent effective against Helicobacter pylori a major pathogen which causes peptic ulcer disease. A sensitive analytical UV spectrophotometric method was developed to quantitate the drug either in bulk or in formulations. Wave length scans of clarithromycin showed its absorption maxima at 211 nm. Linearity studies indicated that estimation of clarithromycin obeyed beers law limit between 2.00 µg /ml to 10.00 µg /ml with regression equation of y = 0.0250*X -0.0010; ( r2 = 0.999). The low SD values of Inter day and Intraday variation studies indicated that the variation is minimum. Limit of Quantitation of clarithromycin was found to be of 1.5 µg /ml. Hence the above analytical parameters indicated that the developed UV Spectrophotometric method of clarithromycin was sensitive, accurate and reproducible. Preformulation studies showed that clarithromycin melting point as 222.6°C, the solubility of drug followed the order acetone > ethanol >methanol > pH 1.2 > pH 4.0 > pH 7.4 > pH 9.0. Further the log p value of clarithromycin was observed as 1.170. The method was found to be precise and can be applied for routine analysis of drug in different pharmaceutical dosage forms and also for dissolution studies.
KEYWORDS:
Clarithromycin, UV Spectrophotometric Method, Validation, Preformulation studies.
Cite:
Putta Rajesh Kumar, Somashekar Shyale, M Mallikarjuna Gouda, SM Shanta Kumar. A Sensitive UV Spectrophotometric Analytical Method Development, Validation and Preformulation Studies of Clarithromycin. Research J. Pharm. and Tech. 4(2): February 2011; Page 242-246.
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