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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   4

Issue No. :  2

Year :  2011

Pages :   230-233

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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TLC Densitometric Methods for Quantification of Gallic Acid in Triphala Churna for Routine Quality Control

Address:   Vishal Jain*, Ambar Vyas, Swarnlata Saraf and S. Saraf
Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur (CG).
*Corresponding Author
DOI No: Not Available

Quantification of active principles through modern analytical tools is essential for establishing the authenticity, creditability, prescription and usage of Ayurvedic medicines/herbal formulations. Triphala churna is one of the popular Ayurvedic preparations, official in Ayurvedic formulary of India. The present study is an attempt to develop a fingerprint method for Triphala churna with TLC Densitometric Methods (HPTLC) using gallic acid as a standard. The gallic acid is a major content in all the three ingredients of the formulation. The method was validated for linearity, accuracy, limit of detection, limit of quantification, inter-day and intra-day assay precision, repeatability of measurement, and repeatability of sample application. The concentration of gallic acid present in raw material was found to be 3.10±0.41% w/w in Emblica officinalis, 8.47±0.82% w/w in Terminalia belerica, and 4.63±0.49% w/w in Terminalia chebula. Gallic acid content in three identical laboratory batch of Triphala churna TP-I, TP-II and TP-III, was found to be 5.39±0.48%, 5.42±0.46% and 5.41±0.52% w/w respectively with mean value 5.41±0.49%. The gallic acid content in all the three different batches is found to be in close proximities with each other. The results were comparable to marketed formulations.
Drotaverine (DRT), Etoricoxib (ETR), Ratio derivative, absorption corrected.
Vishal Jain, Ambar Vyas, Swarnlata Saraf, S Saraf. TLC Densitometric Methods for Quantification of Gallic Acid in Triphala Churna for Routine Quality Control. Research J. Pharm. and Tech. 4(2): February 2011; Page 230-233.
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