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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   4

Issue No. :  10

Year :  2011

Pages :   1563-1566

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Reversed-Phase Liquid Chromatographic Method for Simultaneous Determination of Artesunate and Mefloquine in Pharmaceutical Preparations



Address:   T.M. Kalyankar1* and R.B.Kakde2.
1School of Pharmacy, S.R.T.M. University, Nanded, Maharashtra, India.
2Departments of Pharmaceutical Sciences, R.T.M. Nagpur University, Nagpur, Maharashtra, India.
*Corresponding Author
DOI No: Not Available

ABSTRACT:
A simple, rapid, precise and accurate reversed phase high performance liquid chromatographic method has been developed for the simultaneous determination of Artesunate in combination with Mefloquine. This method uses a Hypersil ODS C18 (250mm×4.6mm×5µ particle Size) analytical column, a mobile phase of Methanol, Acetonitrile and 0.1% Acetic acid in the ratio 70:10:20 (v/v), and pH 4.5 adjusted with orthophosphoric acid. The instrumental settings are a flow rate of 1 ml/min and PDA detector wavelength at 210-360 nm. The retention times for Artesunate and Mefloquine are 6.57 min and 2.19min, respectively. The method is validated and shown to be linear. The linearity range for Artesunate and Mefloquine are 250-1500 and 500-3000 µg/ml respectively. The Percentage recovery for Artesunate and Mefloquine are 99.60-99.35 and 99.49-99.78 respectively. The correlation coefficients of Artesuante and Mefloquine are 0.999, and 0.999, respectively. The relative standard deviation for six replicates is always less than 2%. The Statistical analysis proves that the method is suitable for analysis of Artesunate and Mefloquine as a bulk drug and in pharmaceutical formulation without any interference from the excipients.
KEYWORDS:
Artesunate, Mefloquine, RP-HPLC and validation.
Cite:
T.M. Kalyankar, R.B.Kakde. Reversed-Phase Liquid Chromatographic Method for Simultaneous Determination of Artesunate and Mefloquine in Pharmaceutical Preparations. Research J. Pharm. and Tech. 4(10): Oct. 2011; Page 1563-1566.
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