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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   4

Issue No. :  1

Year :  2011

Pages :   52-56

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Determination and Pharmacokinetics of Ibuprofen in Rabbit Plasma by GC-MS Method



Address:   Bilal Yilmaz1 and Vedat Akba2
1Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, 25240, Erzurum, Turkey
2Criminal Police Laboratory, 25060, Erzurum, Turkey
*Corresponding Author
DOI No: Not Available

ABSTRACT:
This paper describes a gas chromatography-mass spectrometry (GC-MS) method for determination of ibuprofen in rabbit plasma. Ibuprofen and internal standard (IS) naproxen were extracted from plasma by using liquid-liquid extraction method. The samples were separated by GC on a DB-5MS analytical column and determined by a quadrupole mass spectrometer detector operated under selected ion monitoring mode (SIM). Excellent linearity was found between 0.05 and 5.0 µg/mL (r=0.998) for plasma samples. Intra-day and inter-day precisions expressed as the relative standard deviation (RSD) for the method were 3.29-5.14% and 4.26-5.84%, respectively. The mean recovery of ibuprofen samples was 92.03%. This assay was successfully applied to a pharmacokinetic study of ibuprofen in New Zealand white rabbits. As a result, the plasma half-life was 2.68 ± 0.920 h and the mean AUC0-12 h was 187.24 ± 22.42 µg/mL h. The maximum plasma concentration (Cmax) of 59.8 ± 7.416 µg/mL reached 2.0 ± 0.288 h after administration.
KEYWORDS:
Ibuprofen, GC-MS, Liquid-liquid extraction, Pharmacokinetics.
Cite:
Bilal Yilmaz, Vedat Akba. Determination and Pharmacokinetics of Ibuprofen in Rabbit Plasma by GC-MS Method. Research J. Pharm. and Tech. 4 (1): January 2011; Page 52-56.
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