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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   4

Issue No. :  1

Year :  2011

Pages :   63-65

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Estimation of Efavirenz in Tablet Dosage Forms by RP-HPLC.



Address:   Prashanth R1*, V. Kiran Kumar2 and N. Appala Raju3
1Department of Pharmaceutical Analysis, Sree Chaitanya Institute of Pharmaceutical, Sciences, LMD. Colony, Karimnagar. AP.
2Department of Pharmaceutical Analysis, Shantha Institute of Pharmaceutical, Sciences, Huzurabad, (Dist) Karimnagar. AP.
3Department of Pharmaceutical Chemistry, Sultan-Ul-Uloom College of Pharmacy, Mount Pleasant, Road # 3, Banjara Hills, Hyderabad-500 034.
*Corresponding Author
DOI No: Not Available

ABSTRACT:
A simple, precise, rapid and accurate reverse phase HPLC method in isocratic mode has been developed for the estimation of Efavirenz in tablet dosage form. A Hypersil BDS C18, 250x4.6 mm, 5 µm partical size, with mobile phase consisting of acetonitrile and 0.03 M KH2PO4 in water (pH adjusted to 3.2 with orthophosphoric acid) in the ratio of 60:40 v/v was used. The flow rate was 0.8 ml/min and the effluents were monitored at 260 nm. The retention time was 10.549 min for Efavirenz. The detector response was linear for Efavirenz are in the range of 12-144 mcg/ml respectively. The respective linear regression equation being Y=19958.961x+23536.8626 is for Efavirenz. The limit of detection (LOD) for 0.12µg/ml and the limit of quantification (LOQ) for Efavirenz were found to be 0.36µg/ml respectively. The percentage assay of Efavirenz was 98.28%. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Efavirenz in bulk drug and in its pharmaceutical dosage form.
KEYWORDS:
Efavirenz, RP-HPLC, Estimation, and Tablets.
Cite:
Prashanth R, V Kiran Kumar, N Appala Raju. Estimation of Efavirenz in Tablet Dosage Forms by RP-HPLC. Research J. Pharm. and Tech. 4 (1): January 2011; Page 63-65.
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