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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   3

Issue No. :  4

Year :  2010

Pages :   1106-1108

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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High Performance Thin Layer Chromatographic Determination of Spironolactone and Torsemide in Combined Tablet Dosage Form



Address:   Nilesh V. Gaikwad, Padmanabh B. Deshpande*, Santosh V. Gandhi* and Kapil K. Khandagale
Dept. of Pharmaceutical Analysis, AISSMS College of Pharmacy, Kennedy Road, Near R. T. O., Pune – 411 001
*Corresponding Author
DOI No:

ABSTRACT:
A new simple High Performance Thin Layer Chromatographic (HPTLC) method for determination of Spironolactone and Torsemide in combined tablet dosage form has been developed and validated. The mobile phase selected was n-Hexane: Ethyl acetate: Methanol: Glacial acetic acid (7: 3: 1.5: 0.5 v/v/v) with UV detection at 263 nm. The retention factor for SPL and TSM were found to be 0.67 ± 0.03 and 0.34 ± 0.03. The method was validated with respect to linearity, accuracy, precision and robustness. Results found to be linear in the concentration range of 100-1000 ng/band for SPL and TSM respectively. The method has been successfully applied for the analysis of drugs in pharmaceutical formulation. The % assay (Mean ± S.D.) was found to be 99. 75 ± 0.134 for SPL and 98.67 ± 0.153 for TSM.
KEYWORDS:
Spironolactone, Torsemide, High Performance Thin Layer chromatography.
Cite:
Nilesh V. Gaikwad, Padmanabh B. Deshpande, Santosh V. Gandhi, Kapil K. Khandagale. High Performance Thin Layer Chromatographic Determination of Spironolactone and Torsemide in Combined Tablet Dosage Form. Research J. Pharm. and Tech.3 (4): Oct.-Dec.2010; Page 1106-1108.
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