2nd International Conference on Fostering Interdisciplinary Research In Health Sciences (ICFIRHS) 2019 (01-May-2019)        |

Journal :   Research Journal of Pharmacy and Technology

Volume No. :   3

Issue No. :  4

Year :  2010

Pages :   1058-1060

ISSN Print :  0974-3618

ISSN Online :  0974-360X


Registration

Allready Registrered
Click to Login

Validated HPLC Method for Determination of Contents of Residual Aminoguanidine in Drug Substances



Address:   Srivastava Bimal Kumar*, Kushwah Dharmendra Kumar, Kolhe Prakash Yashwant and Patel Nitin
CTX Life Sciences Pvt. Ltd., 251-252, Sachin Magdalla Road, Sachin, Surat, Gujarat-394230, India
*Corresponding Author
DOI No:

ABSTRACT:
This paper describes a validated method for the quantification of aminoguanidine in drug substances and their intermediates using High Performance Liquid Chromatography (HPLC), with a per column derivatization with 1-Naphthyl isothiocyanate. Derivative formed was analysed using a RP-18 HPLC column with pH 3 ortho-phosphoric acid and triethyleamine buffer and Methanol as mobile phase with a gradient elution. Wavelength 220 nm was used for detection. The method is validated for its Specificity, Precision, Accuracy, Linearity Ruggedness and Robustness. Aminoguanidine is linear from 0.015µg/ml to 0.750 µg/ml with correlation coefficient 0.99609. Limit of Quantitation of the method is 0.015 µg/ml and Limit of Detection is 0.010 µg/ml.
KEYWORDS:
Thiourea derivative, HPLC, UV-Vis detection, RP-18 column, 1-Naphthyl isothiocyanate, Limit of Quantitation, and Limit of Detection.
Cite:
Srivastava Bimal Kumar, Kushwah Dharmendra Kumar, Kolhe Prakash Yashwant, Patel Nitin. Validated HPLC Method for Determination of Contents of Residual Aminoguanidine in Drug Substances. Research J. Pharm. and Tech.3 (4): Oct.-Dec.2010; Page 1058-1060.
[View HTML]      [View PDF]



Visitor's No. :   646952