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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   3

Issue No. :  3

Year :  2010

Pages :   847-853

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Quantification of Levetiracetam in Human Plasma with Precipitation Extraction by Using Fully Validated LC-MS/MS and Its Application to a Bioequivalence Study



Address:   Y.K. Naidu1, Raghunadha Reddy S.2*, Koteswara Rao Divi2, M.P. Kalyan Reddy3, I. Sarath Chandiran2 and K.N. Jayaveera3
1Actimus Biosciences (I) Pvt. Ltd., Visakhapatnam, Andhra Pradesh, India.
2Gokula Krishna College of Pharmacy, Sullurpet, Andhra Pradesh, India
3Jawaharlal Nehru Technological University, Anantapoor, Andhra Pradesh, India
*Corresponding Author
DOI No: Not Available

ABSTRACT:
A simple, sensitive and selective method for the determination of Levetiracetam by using rapid high-performance liquid chromatography/positive electroscopy ionization tandem mass spectroscopy was developed. The method consists of precipitation extraction with Acetonitrile followed by the analysis of the extracted sample by liquid chromatography-mass spectroscopy (LC-MS/MS) in selective reaction monitoring mode using electrospray ionization mode (ESI). Chromatography was performed on a C18 reverse phase column, Methanol: Acetonitrile: 10mM ammonium acetate (45:45:10) as a mobile phase. The assay exhibited a linear dynamic range of 1 to 40µg/ml for Levetiracetam in human plasma. Stability assessment was also included. A run time of 3.0 min for each sample made it possible to analyse healthy volunteers participating in pharmacokinetics drug-drug interaction studies.
KEYWORDS:
LC-MS/MS, Human Plasma, Bioanalytical, Levetiracetam, Validation.
Cite:
YK Naidu, Raghunadha Reddy S, Koteswara Rao Divi, MP Kalyan Reddy, I Sarath Chandiran, KN Jayaveera. Quantification of Levetiracetam in Human Plasma with Precipitation Extraction by Using Fully Validated LC-MS/MS and Its Application to a Bioequivalence Study. Research J. Pharm. and Tech.3 (3): July-Sept. 2010; Page 847-853.
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