List of Refereed Journals published by UGC, New Delhi on 28-03-2017-- AJRC (Sr. No.-1025); AJM (Sr. No.-2497); RJET (Sr. No.-7220); RJPP (Sr. No.- 7232): RJPPD (Sr. No.- 7234); RJPT (Sr. No.-7235) (19-Apr-2017)        | List of Refereed Journals published by UGC, New Delhi on 11-01-2017-- Research Journal of Pharmacy and Technology (Sr. No.-275); Asian Journal of Management (Sr. No.-3986); Asian Journal of Research in Chemistry (Sr. No.-4008); Research Journal of Engineering and Technology (Sr. No.-31787); Research (22-Jan-2017)        |

Journal :   Research Journal of Pharmacy and Technology

Volume No. :   3

Issue No. :  3

Year :  2010

Pages :   

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Method Development and Validation of RP-HPLC Method for Estimation of Nateglinide in Bulk Drug and Pharmaceutical Formulation.

Address:   Department of Pharmaceutical Analysis, C. L. Baid Metha College of Pharmacy, Jyothinagar, Thoraipakkam .Chennai-600 097, Tamilnadu, India.
DOI No: Not Available

A rapid, simple, precise and sensitive RP-HPLC method was developed for estimation of nateglinide in pharmaceutical bulk drug and pharmaceutical formulation. A phenomenex Gemini C18, 5µm particle size column having 250 x 4.6 mm .i.d in isocratic mode, with mobile phase containing acetonitrile: 0.1%w/v sodium dihydrogen ortho phosphate (70:30v/v) was used. The flow rate was 2ml/min and the effluent was monitored at 220nm. The retention time of nateglinide was 6.7±0.2 minutes. The method was validated for linearity, accuracy, precision, specificity, LOD, LOQ and robustness. Nateglinide showed linear response in the concentration range 160-240µg/ml. the correlation coefficient for nateglinide was 0.9999 and the percentage recovery was found to be 99.26to 100.81.
Nateglinide, bulk drug, pharmaceutical formulation, RP-HPLC.
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