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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   3

Issue No. :  3

Year :  2010

Pages :   773-775

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Validated RP- HPLC Method for the Quantitative Estimation of Tramadol in Bulk and Pharmaceutical Dosage Forms



Address:   C.H. Sharada*, K.P. Channabasavaraj, Srikanth A., Jose Gnana Babu, V. Reddy Panditi and Y. Bhargava Reddy
Dept. of Pharmaceutical Analysis, Bharathi College of Pharmacy, Bharathi Nagara, Mandya District, Karnataka, India – 571 422.
*Corresponding Author
DOI No: Not Available

ABSTRACT:
A simple, specific, accurate, precise and sensitive reverse phase High Performance Liquid Chromatographic method has been developed for the quantitative estimation of Zidovudine in both pure and pharmaceutical dosage forms. A Venusil XBP C- 18, 5 µm column having 250 × 4.6 mm internal diameter in isocratic mode with mobile phase containing Water: Methanol (50:50). The flow rate was 1.0 ml/min and the effluents were monitored at 266 nm. The retention time was 4.47 min. The linearity was in the range of 25-75 µg/ml. This method was validated for linearity, precision, limit of detection, limit of quantitation, accuracy, ruggedness and robustness. Statistical analysis proves that the method is reproducible and selective for the estimation of the said drug.
KEYWORDS:
RP-HPLC, Tramadol, Validation.
Cite:
Y Bhargava Reddy, C Jose Gnana Babu, KP Channabasavaraj, CH Sharada, V Reddy Panditi. Validated RP- HPLC Method for the Quantitative Estimation of Tramadol in Bulk and Pharmaceutical Dosage Forms. Research J. Pharm. and Tech.3 (3): July-Sept. 2010; Page 773-775.
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