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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   3

Issue No. :  3

Year :  2010

Pages :   

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Validated RP- HPLC Method for the Quantitative Estimation of Tramadol in Bulk and Pharmaceutical Dosage Forms



Address:   Department of Pharmaceutical Analysis, Bharathi College of Pharmacy, Bharathi Nagara, Mandya District, Karnataka, India – 571 422.
DOI No: Not Available

ABSTRACT:
A simple, specific, accurate, precise and sensitive Reverse Phase High Performance Liquid Chromatographic method has been developed for the quantitative estimation of Tramadol in both pure and pharmaceutical dosage forms. A Phenomenex RP C- 18, 5 µm column having 250 × 4.6 mm internal diameter in isocratic mode with mobile phase containing Triflouroacetic acid: Acetonitrile (81:19). The flow rate was 1.5 ml/min and the effluents were monitored at 270 nm. The retention time was 3.615 min. The linearity was in the range of 0.25 - 0.75 mg/ml. This method was validated for linearity, precision, specificity, limit of detection, limit of quantitation, accuracy, ruggedness and robustness. Statistical analysis proves that the method is reproducible and selective for the estimation of the said drug.
KEYWORDS:
RP-HPLC, Tramadol, Validation.
Cite:
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