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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   3

Issue No. :  3

Year :  2010

Pages :   770-772

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Validated RP- HPLC Method for the Quantitation of Stavudine in Bulk and Pharmaceutical Dosage Forms



Address:   V Reddy Panditi*, C. Jose Gnana Babu, KP Channa Basavaraj, CH Sharada and Y Bhargava Reddy
Department of Pharmaceutical Analysis, Bharathi College of Pharmacy, Bharathi Nagara, Mandya District, Karnataka, India – 571422.
*Corresponding Author
DOI No: Not Available

ABSTRACT:
A simple, specific, accurate, precise and sensitive Reverse Phase High Performance Liquid Chromatographic method has been developed for the quantitation of Stavudine in both pure and pharmaceutical dosage forms. A Venusil XBP C-18, 5 µm column having 250 × 4.6 mm internal diameter in isocratic mode with mobile phase containing 0.01M Ammonium acetate buffer: Methanol (60: 40). The flow rate was 1.0 ml / min and the effluents were monitored at 265 nm. The retention time was 3.65 min. The linearity was in the range of 25-75 mcg / ml. This method was validated for linearity, precision, limit of detection, limit of quantitation, accuracy, ruggedness and robustness. Statistical analysis proves that the method is reproducible and selective for the estimation of the said drug.
KEYWORDS:
RP-HPLC, Stavudine, Validation.
Cite:
V Reddy Panditi, C Jose Gnana Babu, KP Channa Basavaraj, CH Sharada, Y Bhargava Reddy. Validated RP- HPLC Method for the Quantitation of Stavudine in Bulk and Pharmaceutical Dosage Forms. Research J. Pharm. and Tech.3 (3): July-Sept. 2010; Page 770-772.
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