2nd International Conference on Fostering Interdisciplinary Research In Health Sciences (ICFIRHS) 2019 (01-May-2019)        |

Journal :   Research Journal of Pharmacy and Technology

Volume No. :   3

Issue No. :  2

Year :  2010

Pages :   613-618

ISSN Print :  0974-3618

ISSN Online :  0974-360X


Allready Registrered
Click to Login

Optimization of Pharmacokinetic By Loading Bioenhancer in Optimized Nasal Mucoadhesive Microspheres of Insulin

Address:   Shinde AD1* and Bhise SB2
1S.V.P.M.’s College of Pharmacy, Malegaon (Bk II), Baramati, Pune, Maharashtra, India
2Govt. College of Pharmacy, Karad, Dist-Satara, Maharashtra. India
*Corresponding Author
DOI No: Not Available

Mucoadhesive Microspheres of Bovine Insulin containing a muchoadhesive polymers Sodium Alginate and Carbapol-934 were prepared by Ionic Gelation technique and Bovine Insulin encapsulated by lyophilization by utilizing 32 Factorial Drug Design and 9 formulation prepared. The formulation C-3 was selected as a final optimized formulation for its significant characteristics. The Optimized Mucoadhesive microspheres C-3 was loaded with bioenhancers such as Sodium deoxycholate, Sodium Taurocholate and Lecithin from soya in 1% w/w. Drug release study was carried out. The mucoadhesive microspheres loaded with 1% w/w Sodium deoxycholate indicate significant release profile. Hence the final optimized formulation loaded with1% w/w Sodium deoxycholate was investigate for animal study by selecting rabbit model. The diabetes was induced by using Alloxan 150 mg/kg to rabbits. The final optimized formulation loaded with and without 1% w/w Sodium deoxycholate. The formulation without bioenhancer was remarked as reference formulation; both formulations were given to animal by nasal route, with a dose 1.5 U/Kg by selecting Crossover drug design study. Prior to investigate a optimized and reference formulation to animal a standard dose of bovine insulin was given to each animal 0.5 U/Kg by subcutaneously. Serum blood concentration of bovine insulin was estimated by using previously calibrated and validated RP-HPLC method at predetermined time schedule and pharmacokinetic study was done. The % blood glucose levels were recorded during animal study.
Bovine Insulin, Mucoadhesive microsphere, Diabetes, Pharmacokinetic.
Shinde AD, Bhise SB. Optimization of Pharmacokinetic By Loading Bioenhancer in Optimized Nasal Mucoadhesive Microspheres of Insulin. Research J. Pharm. and Tech. 3(2): April- June 2010; Page 613-618.
[View HTML]      [View PDF]

Visitor's No. :   646833