List of Refereed Journals published by UGC, New Delhi on 28-03-2017-- AJRC (Sr. No.-1025); AJM (Sr. No.-2497); RJET (Sr. No.-7220); RJPP (Sr. No.- 7232): RJPPD (Sr. No.- 7234); RJPT (Sr. No.-7235) (19-Apr-2017)        | List of Refereed Journals published by UGC, New Delhi on 11-01-2017-- Research Journal of Pharmacy and Technology (Sr. No.-275); Asian Journal of Management (Sr. No.-3986); Asian Journal of Research in Chemistry (Sr. No.-4008); Research Journal of Engineering and Technology (Sr. No.-31787); Research (22-Jan-2017)        |

Journal :   Research Journal of Pharmacy and Technology

Volume No. :   3

Issue No. :  2

Year :  2010

Pages :   

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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HPTLC Method Development and Validation for the Estimation of Rabeprazole Sodium and Itopride Hydrochloride in Tablet Dosage form

Address:   1K.M.C.H. College of Pharmacy, Kovai Estate, Kalappatti Road, Coimbatore – 641035, Tamilnadu. 2Department of Pharmaceutical Analysis, Donbosco College of pharmacy, Pulladi Gunta, Etukuru,Guntur, A. P.
DOI No: Not Available

The simple, accurate and precise method for the quantitative determination of Rabeprazole sodium (RP) and Itopride hydrochloride (IH), from its tablet dosage form by HPTLC method, the chromatograms were developed using a mobile phase of Ethyl acetate : Methanol : Ammonia ( 8.5:1:0.5 v/v ) on precoated plate of silica gel 60 F254 and quantified by densitometric absorbance mode at 285 nm. The Rf values of IH and RP were 0.21 and 0.41 respectively. Linearity of Itopride hydrochloride (IH) and Rabeprazole sodium (RP) was in the range of 75 - 375 ng /ml and 10 - 50 ng /ml. Recovery studies of 98.88 – 102.41%, percentile relative std deviation of not more than 0.8 and correlation coefficient (linearity range) of 0.9954 – 0.9999 for IH and RP, it shows that developed methods were accurate and precise. The LOD and LOQ values were found to be 10ng/ml, 30ng/ml and 5ng/ml, 10ng/ml for IH and RP respectively. The mean percentage recovery values close to 100% it indicates there is no interferences of additives with Rabeprazole sodium (RP) and Itopride hydrochloride (IH) present in tablet dosage forms. The method has been validated as per ICH guide lines. This method can be employed for the routine analysis of tablets containing IH and RP.
High performance liquid thin layer chromatography (HPTLC); Rabeprazole sodium (RP); Itopride hydrochloride (IH).
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