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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   3

Issue No. :  1

Year :  2010

Pages :   

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Stability Indicating RP-HPLC Method for Analysis of Ciprofloxacin in Tablet Dosage Form



Address:   1Department of Pharmaceutical Chemistry, Himalayan Pharmacy Institute, Majhitar, E.Sikkim-737136. 2Dept. of Pharmaceutics, Calcutta Institute of Pharmaceutical Technology and AHS, Howrah, West Bengal-711316. 3Dept. of Pharmaceutical Analysis, Vaagdevi C
DOI No: Not Available

ABSTRACT:
A simple, sensitive, accurate, reproducible and stability indicating RP-HPLC method was developed for the rapid analysis of Ciprofloxacin in tablet dosage form. The chromatographic separation was performed on a RP C18 (250 x 4.6 i.d) mm, 5µm, column. The mobile phase of the method consists of equal amount of acetonitrile and methanol along with 1% acetic acid in aqueous solution in the ratio of 8:8:84 v/v in the flow rate of 1.5 mL/min. The retention time for ciprofloxacin is found to be 7.7min when monitored at 280 nm. The detector response was linear in the concentration range of 200-700 µg/mL. The limit of detection and limit of quantification was 15.145 and 44.784 µg/mL respectively. The percentage assay of Ciprofloxacin was 99.92%. Correlation coefficient (r) of the regression equation is more than 0.999. The precision of the method was established by intra and inter day assay values which were less than 1%. None of the degraded products interfered with the Ciprofloxacin peak thus ensuring stability indicating nature of the method.
KEYWORDS:
Ciprofloxacin, Stability Indicating, RP-HPLC
Cite:
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