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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   3

Issue No. :  1

Year :  2010

Pages :   165-167

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Antimicrobial Activity of Methanolic Extracts of Mucuna pruriens, Semecarpus anacardium, Anethum graveolens by Agar Disc Diffusion Method



Address:   Sanjay R Patel*, Ashok P Suthar, Anand M Shah, Hitesh V Hirpara, Vishal D Joshi and Mayur V Katheria
KNV Pharmacy College, Metoda, Rajkot, Gujarat, India.
*Corresponding Author
DOI No: Not Available

ABSTRACT:
The investigation is aimed to bring out the antimicrobial actions of the methanol extracts of Mucuna pruriens seeds, Semecarpus anacardium nuts, Anethum graveolens fruits. The antimicrobial activity of the extracts was determined by the agar well diffusion method against various gram positive bacteria, gram negative bacteria. The zone of inhibition of methanolic extract of Mucana pruriens against Bacillus subtilis MTTC 121 (24 mm, 22 mm, 17mm, 14 mm, 10 mm), Staphylococcus aureus MTCC 96 (20 mm, 16 mm, 13 mm, 10 mm, 8 mm), and Escherichia coli MTCC 521 (19 mm, 16 mm, 14 mm, 12 mm, 7 mm) at 10 mg/ml, 5 mg/ml, 2.5 mg/ml, 1 mg/ml, 0.5 mg/ml respectively. The zone of inhibition of methanolic extract of Semecarpus anacardium against Bacillus subtilis MTTC 121 (11 mm, 9 mm, 8 mm, none, none), Staphylococcus aureus MTCC 96 (8 mm, 7 mm, none, none, none), and Escherichia coli MTCC 521 (8 mm, none, none, none, none) at 10 mg/ml, 5 mg/ml, 2.5 mg/ml, 1 mg/ml, 0.5 mg/ml respectively. The zone of inhibition of methanolic extract of Anethum graveolens against Bacillus subtilis MTTC 121 (24 mm, 21 mm, 16 mm, 12 mm, 8 mm), Staphylococcus aureus MTCC 96 (18 mm, 15 mm, 12 mm, 9 mm, none), and Escherichia coli MTCC 521 (25 mm, 21 mm, 16 mm, 12 mm, 8 mm) at 10 mg/ml, 5 mg/ml, 2.5 mg/ml, 1 mg/ml, 0.5 mg/ml respectively. The results obtained within these study shows that Mucana pruriens extract can be a potential source of natural antimicrobial agent than the nuts of Semecarpus anacardium. The fruit of Anethum graveolens has linked similar exploit as per Mucuna pruriens.
KEYWORDS:
Antimicrobial activity, Mucuna pruriens, Semecarpus anacardium, Anethum graveolens, Agar diffusion.
Cite:
Sanjay R Patel, Ashok P Suthar, Anand M Shah, Hitesh V Hirpara, Vishal D Joshi , Mayur V Katheria. Antimicrobial Activity of Methanolic Extracts of Mucuna pruriens, Semecarpus anacardium, Anethum graveolens by Agar Disc Diffusion Method. Research J. Pharm. and Tech. 3(1): Jan. - Mar. 2010; Page 165-167.
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