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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   3

Issue No. :  1

Year :  2010

Pages :   102-105

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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HPLC and Colorimetric Methods for Estimation of Cefepime



Address:   Jane J*, Prashant Kumar D. and EVS Subrahmanyam
NGSM Institute of Pharmaceutical Sciences, Paneer, Deralakatte-574160, Mangalore
*Corresponding Author
DOI No: Not Available

ABSTRACT:
A simple, rapid and economical HPLC method and two colorimetric methods have been developed for the estimation of cefepime in its bulk and Pharmaceutical dosage forms. Method I involved an isocratic elution of Cefepime in a column of Phenomenex C18, 250 X 4.6, 5µ, using a mobile phase composition of Methanol: Water (70:30, v/v). The flow rate was 0.8 ml/min and the analyte was monitored at 250 nm. Method II was based on oxidative coupling reaction between cefepime and MBTH in presence of ferric chloride anhydrous to produce a green colored chromogen with an absorption maximum of 639nm. The chromogen obeyed linearity in the range 2-12 µg/ml. Method III is based on reaction between cefepime and 1, 10 phenanthroline in presence of ferric ammonium sulphate to produce a red color chromogen with absorption maximum of 493nm. The chromogen obeyed linearity in the range 0.5-3µg/ml.
KEYWORDS:
HPLC, Colorimetry, cefepime.
Cite:
Jane J, Prashant Kumar D, EVS Subrahmanyam. HPLC and Colorimetric Methods for Estimation of Cefepime. Research J. Pharm. and Tech. 3(1): Jan.-Mar. 2010; Page 102-105.
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