Journal :   Research Journal of Pharmacy and Technology

Volume No. :   2

Issue No. :  4

Year :  2009

Pages :   746-748

ISSN Print :  0974-3618

ISSN Online :  0974-360X


Registration

Allready Registrered
Click to Login

A Validated High Performance Thin Layer Chromatographic Method for Simultaneous Estimation of Nebivolol Hydrochloride and Valsartan in Pharmaceutical Dosage Form



Address:   Shradhanjali M Singh, Kirti S Topagi, and Mrinalini C Damle*
Dep. of Pharmaceutical Chemistry, A.I.S.S.M.S. College of Pharmacy, Kennedy Road, Near R.T.O., Pune – 411001
*Corresponding Author
DOI No:

ABSTRACT:
A simple, accurate and precise high performance thin layer chromatographic method has been developed for the estimation of Valsartan and Nebivolol hydrochloride simultaneously from a tablet dosage form. The method employed silica gel 60 F254 precoated plates as stationary phase and a mixture of Ethyl acetate: Methanol: Ammonia (6.5:2.5:0.5 %v/v/v) as mobile phase. Densitometric scanning was performed at 280 nm using Camag TLC scanner 3. Beer’s law was obeyed in the concentration range of 800ng/spot-2400ng/spot for Nebivolol hydrochloride and 200ng/spot-1000ng/spot for Valsartan. The Retention factors for Nebivolol hydrochloride is 0.75 ± 0.04 and for Valsartan is 0.27 ± 0.01. The method was validated as per ICH Guidelines, proving its utility in estimation of Valsartan and Nebivolol hydrochloride in combined dosage form.
KEYWORDS:
A Validated High Performance Thin Layer Chromatographic Method for Simultaneous Estimation of Nebivolol Hydrochloride and Valsartan in Pharmaceutical Dosage Form.
Cite:
Shradhanjali M Singh, Kirti S Topagi, Mrinalini C Damle. A Validated High Performance Thin Layer Chromatographic Method for Simultaneous Estimation of Nebivolol Hydrochloride and Valsartan in Pharmaceutical Dosage Form. Research J. Pharm. and Tech.2 (4): Oct.-Dec. 2009; Page 746-748.
[View HTML]      [View PDF]



Visitor's No. :   376426