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Journal :   Research Journal of Pharmacy and Technology

Volume No. :   2

Issue No. :  4

Year :  2009

Pages :   

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Reverse Phase - High Performance Liquid Chromatography Method for the Analysis of Paracetamol

Address:   University Dept. of Pharmaceutical Sciences, M.L.S. University, Udaipur, Rajasthan
DOI No: Not Available

An accurate, simple, reproducible and sensitive method for the determination of paracetamol was developed and validated. Paracetamol was identified using a , Microsorb-MV 100-5 C18 column (250 x 4.6 mm; North America) by binary gradient elution with a flow rate of 0.5 ml/min. The mobile phase composition was methanol: water (30:70) (v/v) and spectrophotometric detection was carried out at 245 nm. The linear range of determination for paracetamol was 0.816-250 μg/ml. The method was shown to be linear, reproducible, specific and sensitive.
Paracetamol, Reverse phase-High performance liquid chromatography, Validation.
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