List of Refereed Journals published by UGC, New Delhi on 28-03-2017-- AJRC (Sr. No.-1025); AJM (Sr. No.-2497); RJET (Sr. No.-7220); RJPP (Sr. No.- 7232): RJPPD (Sr. No.- 7234); RJPT (Sr. No.-7235) (19-Apr-2017)        | List of Refereed Journals published by UGC, New Delhi on 11-01-2017-- Research Journal of Pharmacy and Technology (Sr. No.-275); Asian Journal of Management (Sr. No.-3986); Asian Journal of Research in Chemistry (Sr. No.-4008); Research Journal of Engineering and Technology (Sr. No.-31787); Research (22-Jan-2017)        |

Journal :   Research Journal of Pharmacy and Technology

Volume No. :   2

Issue No. :  3

Year :  2009

Pages :   

ISSN Print :  0974-3618

ISSN Online :  0974-360X


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Quantitation of Valsartan in Human Plasma by High Performance Liquid Chromatography with Fluorescence Detection and its Application to Bioequivalence Study

Address:   Shri C.B. Patel Research Centre, 3rd Floor, Bhaidas Hall Bldg, JVPD Scheme, Vile-Parle (West), Mumbai 400056
DOI No: Not Available

A selective, accurate and precise high-performance liquid chromatographic method was developed for the quantitation of an angiotensin II receptor antagonist Valsartan in human plasma. HPLC separation was performed on a reversed phase Inertsil ODS-3V, C18 (250x4.6mm, 5µ) column, using an isocratic mobile phase of Acetonitrile : Water (60 : 40, v/v) containing 0.1% Triethylamine and pH was adjusted to 3.5 with 10% orthophosphoric acid. The peak response in terms of peak area was measured with fluorescence detector, set at an excitation wavelength 250 nm and emission wavelength 371 nm, at room temperature. The method was validated in terms of linearity, accuracy, precision, recovery, stock solution, freeze-thaw cycle, autosampler stability and bench top stability. Linearity was observed over a range of 20-1500 ng/mL. The validated method was successfully applied for the analysis of plasma samples from a Pharmacokinetic study.
Valsartan, Fluorescence, Pharmacokinetics.
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