List of Refereed Journals published by UGC, New Delhi on 28-03-2017-- AJRC (Sr. No.-1025); AJM (Sr. No.-2497); RJET (Sr. No.-7220); RJPP (Sr. No.- 7232): RJPPD (Sr. No.- 7234); RJPT (Sr. No.-7235) (19-Apr-2017)        | List of Refereed Journals published by UGC, New Delhi on 11-01-2017-- Research Journal of Pharmacy and Technology (Sr. No.-275); Asian Journal of Management (Sr. No.-3986); Asian Journal of Research in Chemistry (Sr. No.-4008); Research Journal of Engineering and Technology (Sr. No.-31787); Research (22-Jan-2017)        |

Journal :   Research Journal of Pharmacy and Technology

Volume No. :   2

Issue No. :  3

Year :  2009

Pages :   

ISSN Print :  0974-3618

ISSN Online :  0974-360X


Allready Registrered
Click to Login

Validated RP-HPLC Method for Simultaneous Determination of Amlodipine Besylate and Hydrochlorothiazide from Pharmaceutical Preparation and Biological Samples

Address:   Bharati Vidyapeeth College of Pharmacy, Near Chitranagari, Morewadi , Kolhapur
DOI No: Not Available

A simple sensitive, rapid, accurate and precise reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of amlodipine besylate and hydrochlorothiazide from pharmaceutical preparation, blood sample and urine The method involves HIQ Sil C18 5µm column (250 mm x 4.6 mm i.d.) using pphosphate buffer pH 3.5: Acetonitrile (60:40V/V) as mobile phase at a flow rate of 1ml/min with wavelength detection at 240nm. Losartan potassium was used as an internal standard. The retention time of hydrochlorothiazide, amlodipine besylate and Losartan potassium were 3.7, 5.5 and 8.7 ±0.02 minutes respectively. The developed method was applied for simultaneous estimation of the two drugs from pharmaceutical formulation, plasma and urine and was validated according to ICH and USFDA guidelines. The recovery of both the drugs was found to be greater than 98% and % RSD did not exceed 2% indicating high degree of accuracy of the proposed HPLC method.
Amlodipine besylate; hydrochlorothiazide; losartan potassium; plasma; urine.
Not Available
[View HTML]     

Visitor's No. :   174279